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Bcui Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638369

MBS638369 is a restriction enzyme targeting BcuI. Also known as BcuI (SpeI). Host: Bacillus coagulans VS 29-022. 5'-A^C T A G T-3' More details

$475 / 400 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BcuI, Restriction Enzyme
Full Product Name
BcuI (SpeI)
Host
Bacillus coagulans VS 29-022
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used.
Digestion of Agarose-embedded DNA
A minimum of 10units of enzyme is required for digestion of 1ug of agarose-embedded Adenovirus-2 DNA in 16 hours.
Compatible Ends
Eco130I, NheI, XbaI, XmaJI
pACYC177
0
pACYC184
0
Blue/White Cloning Assay
The mix of pUC57/HindIII, pUC57/PstI and pUC57/Eco32I digests was incubated with 10units of enzyme for 16 hours. After religation and transformation 1.1% of white colonies were detected.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda
0
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with BcuI, more than 80% of the DNA fragments can be ligated at a 5'-termini concentration of 0.05uM. More than 90% of these can be recut.
M13mp18/19
0
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug Ad2 DNA x 16 hours) with BcuI.
pBR322
0
PhiX174
0
pTZ19R/U
0
pUC18/19
0
pUC57
0
Stability During Prolonged Incubation
A minimum of 0.5units of enzyme is required for complete digestion of 1ug of Ad2 DNA in 16 hours at 37 degree C.
Storage Buffer
10mM Tris-HCl (pH 7.5 at 25 degree C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Enzyme is not inactivated by incubation at 80 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of AD2 DNA fragments in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for BcuI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of BcuI restriction enzyme

Restriction Enzyme – Cat# MBS638369

Other Notes

Small volumes of BcuI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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