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Cfr42i Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638387

MBS638387 is a restriction enzyme targeting Cfr42I. Also known as Cfr42I (SacII). 5'-C C G C^G G-3' More details

$440 / 1200 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cfr42I, Restriction Enzyme
Full Product Name
Cfr42I (SacII)
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used.
Digestion of Agarose-embedded DNA
A minimum of 20units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends
Bsh1285I
pACYC177
1
pACYC184
1
Blue/White Cloning Assay
The mix of pUC57/HindIII, pUC57/PstI and pUC57/Eco32I digests was incubated with 10units of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda
4
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with Cfr42I, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.05uM. More than 95% of these can be recut.
M13mp18/19
0
Note
Certain sites in lambda and PhiX174 DNAs are difficult to cleave with Cfr42I, as well as with its prototype SacII.
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 240-fold overdigestion (15u/ug lambda DNA x 16 hours) with Cfr42I.
pBR322
0
PhiX174
1
pTZ19R/U
0
pUC18/19
0
pUC57
0
Stability During Prolonged Incubation
A minimum of 0.1units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer
10mM potassium phosphate (pH 7.4 at 25 degree C), 100mM NaCl, 1mM EDTA, 7mM 2-mercaptoethanol, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for Cfr42I restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of Cfr42I restriction enzyme

Restriction Enzyme – Cat# MBS638387

Other Notes

Small volumes of Cfr42I restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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