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Mnl I Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638388

MBS638388 is a restriction enzyme targeting Mnl I. 5'...C C T C (N)7...3' More details

$470 / 300 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mnl I, Restriction Enzyme
Full Product Name
Mnl I
Form/Format
10mM Tris-HCl, pH 7.4 at 25 degree C, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplied With
R1625: Restriction Enzyme Buffer A, 10X
R1625-02: Restriction Enzyme Buffer C, 10X
Note: R1625-02 gives 100% digestion where R1625 will only give ~20-50% digestion at the same concentrati
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda
262
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with Mnl I, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 2uM. More than 90% of these can be recut.
M13mp18/19
61
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Mnl I.
pBR322
26
PhiX174
34
pTZ19R/U
12
pUC18/19
13
pUC57
14
Stability During Prolonged Incubation
A minimum of 0.5units of enzyme are required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Thermal Inactivation
Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for Mnl I restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of Mnl I restriction enzyme

Restriction Enzyme – Cat# MBS638388

Other Notes

Small volumes of Mnl I restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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