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Pvu II Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638393

MBS638393 is a restriction enzyme targeting Pvu II. Host: Proteus vulgaris. 5'-C A G^C T G-3' More details

$430 / 2500 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Pvu II, Restriction Enzyme
Full Product Name
Pvu II
Host
Proteus vulgaris
Purity/Purification
Molecular Biology Grade
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Stable for at least 12 months
Supplied With
R1625 Restriction Enzyme Buffer A, 10X
Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of PvuI for 4 hours.
Lambda
15
Ligation/Recutting Assay
After 10-fold overdigestion (5u/ug DNA x 2 hours) with PvuII, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.06uM. More than 95% of these can be recut.
M13mp18/19
3 pBR322: 1
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 10-fold overdigestion (15u/ug lambda DNA x 1 hours) with PvuII (see Star Activity).
pTZ19R/U
2
pUC18/19
2 pUC57 : 2
Stability During Prolonged Incubation
A minimum of 0.2units of PvuI are required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Unit Definition
One unit is defined as the amount of PvuI required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for Pvu II restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of Pvu II restriction enzyme

Restriction Enzyme – Cat# MBS638393

Other Notes

Small volumes of Pvu II restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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