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Bspti Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638396

MBS638396 is a restriction enzyme targeting BspTI. Also known as BspTI (AflII). Host: Bacillus species RFL1265I. 5'-C^T T A A G-3' More details

$445 / 1000 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BspTI, Restriction Enzyme
Full Product Name
BspTI (AflII)
Host
Bacillus species RFL1265I
Form/Format
Supplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25 degree C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
R1625: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or a Storage Buffer.
Digestion of Agarose-embedded DNA
A minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends
SmlI
pACYC177
0
pACYC184
0
Blue/White Cloning Assay
The mix of pUC57/HindIII, pUC57/PstI and pUC57/Eco32I digests was incubated with 10units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda
3
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with BspLI, approximately 80% of the DNA fragments can be ligated at a 5'-termini concentration of 0.2uM. More than 90% of these can be recut.
M13mp18/19
0
pBR322
0
PhiX174
2
pTZ19R/U
0
pUC18/19
0
pUC57
0
Stability During Prolonged Incubation
A minimum of 0.1units of enzyme is required for complete digestion of 1ug of DNA in 16 hours at 37 degree C.
Thermal Inactivation
Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dam-in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for BspTI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of BspTI restriction enzyme

Restriction Enzyme – Cat# MBS638396

Other Notes

Small volumes of BspTI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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