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Bcni Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638402

MBS638402 is a restriction enzyme targeting BcnI. Also known as BcnI (CauII). Host: Bacillus centrosporus RFL1. G More details

$430 / 1000 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BcnI, Restriction Enzyme
Full Product Name
BcnI (CauII)
Host
Bacillus centrosporus RFL1
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used.
Compatible Ends
Bme1390I, SatI.
Methylation Effects
Sequence 5'…Cm5CGGG…3' 3'…GGm5CCC…5' is cleaved by BcnI at a significantly slower rate. CG methylation may influence DNA cleavage.
pACYC177
6
pACYC184
10
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda
114
Ligation/Recutting Assay
After 10-fold overdigestion (2u/ug DNA x 5 hours) with BcnI, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5'-termini concentration of 0.03uM. More than 95% of these can be recut.
M13mp18/19
4
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BcnI.
pBR322
10
PhiX174
1
pTZ19R/U
5
pUC18/19
7
pUC57
7
Stability During Prolonged Incubation
A minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer
10mM potassium phosphate (pH 7.4 at 25 degree C), 200mM NaCl, 1mM EDTA, 7mM 2-mercaptoethanol, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for BcnI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of BcnI restriction enzyme

Restriction Enzyme – Cat# MBS638402

Other Notes

Small volumes of BcnI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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