Mbo I (mbo) Restriction Enzyme

Name: mbo Restriction Enzyme; Mbo I; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS638410
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638410

MBS638410 is a restriction enzyme targeting mbo. Also known as Mbo I. Host: Moraxella bovis. 5'-^G A T C-3' More details

$480 / 300 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Mbo I, Restriction Enzyme

Full Product Name

Mbo I

Product Gene Name

mbo restriction enzyme

Host

Moraxella bovis

Purity/Purification

Molecular Biology Grade

Preparation and Storage

-20 degree C

Molecular Weight

78,681 Da

Chromosome Location

Chromosome: 3R; NT_033777.2 (8502209..8504774, complement). Location: 87C5-87C5

3D Structure

ModBase 3D Structure for Q9GYU8

Dilution Buffer

10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods the Storage Buffer should be used.

Compatible Ends

BamHI, BclI, BglII, PsuI

Methylation Effects

MboI cuts neither Gm6ATC, GAThm5C nor GAhm5UC, but cuts GATm4C and GATm5C. Blocked by Dam methylation.

pACYC177

pACYC184

Labeled Oligonucleotide (LO) Assay

No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.

Lambda

116

Ligation/Recutting Assay

After 50-fold overdigestion (3u/ug DNA x 17 hours) with MboI, more than 90% of the DNA dam-, fragments can be ligated at a 5'-termini concentration of 1.2uM. More than 90% of these can be recut.

M13mp18/19

Overdigestion Assay

No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA dam-x 16 hours) with MboI.

pBR322

PhiX174

pTZ19R/U

pUC18/19

pUC57

Stability During Prolonged Incubation

A minimum of 0.1units of enzyme are required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.

Storage Buffer

10mM Tris-HCl (pH 7.5 at 25 degree C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.

Thermal Inactivation

Enzyme is inactivated by incubation at 65 degree C for 20min.

Unit Definition

One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dam-in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for mbo restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Other Notes

Small volumes of mbo restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for mbo. It may not necessarily be applicable to this product.

NCBI GI #

24646365

NCBI GeneID

41562

NCBI Accession #

NP_524330.2

UniProt Primary Accession #

Q9GYU8

UniProt Secondary Accession #

Q9VG41

UniProt Related Accession #

Q9GYU8

GenBank Nucleotide #

NM_079606.4

NCBI Official Full Name

members only

NCBI Official Synonym Full Names

members only

NCBI Official Symbol

mbo

NCBI Official Synonym Symbols

1465/07; 1465/7; anon-WO0118547.263; CG6819; DmelCG6819; Dnup 88; dNup88; Dnup88; DNup88; l(3)05043; l(3)S146507; Mbo; nup88; Nup88

UniProt Protein Name

Nuclear pore complex protein Nup88

UniProt Synonym Protein Names

88 kDa nuclear pore complex protein; Nucleoporin Nup88; Protein members only; dNup88

Protein Family

Nuclear pore complex protein

UniProt Gene Name

mbo

UniProt Synonym Gene Names

Nup88

UniProt Entry Name

NUP88_DROME

NCBI Protein Information

CG6819 gene product from transcript CG6819-RA; CG6819-PA; mbo-PA; members only

UniProt Comments for mbo

Function: Essential component of nuclear pore complex. Selectively required for the nuclear import of the Rel family transcription factors dorsal (dl) and Dorsal-related immunity factor (Dif) and the activation of an immune response. Required for the anchoring of Nup214 and emb on the nuclear envelope and thereby attenuates nuclear export signal (NES)-mediated nuclear export. Ref.1 Ref.5

Subunit structure: Interacts with Nup214 and emb to form components of the nuclear pore complex. Interacts with tamo for nuclear import of target proteins including dl, msl-1, ran and ran-like. Ref.5 Ref.6

Subcellular location: Nucleus › nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side Ref.1.

Tissue specificity: In the embryo, expression is prominent in a subset of the cells of the developing CNS, dynamic stripes of epidermal cells, lymph glands and intestinal tract including the proventriculus and foregut. In the larva, expression was seen in the fusion cells of the trachea, fat body, imaginal tissues, and with abundance in the proliferating parts of the nerve cord, optic lobes of the brain and imaginal disks. Ref.1

Developmental stage: Expressed both maternally and zygotically. Ref.1

Research Articles on mbo

1. Nup88 localizes to silent loci, Sec13, Nup98, and a subset of FG-repeat nucleoporins bind to developmentally regulated genes undergoing transcription induction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with mbo restriction enzyme

Reference Product	PubMed Publications
Nup214 restriction enzyme	>2 publications with mbo and Nup214
Nup98 restriction enzyme	>1 publications with mbo and Nup98

Pathways associated with mbo restriction enzyme

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Metabolism Of Non-coding RNA Pathway antibodies	Metabolism Of Non-coding RNA Pathway Diagram
Mitotic Prophase Pathway antibodies	Mitotic Prophase Pathway Diagram
Nuclear Envelope Breakdown Pathway antibodies	Nuclear Envelope Breakdown Pathway Diagram
Nuclear Pore Complex (NPC) Disassembly Pathway antibodies	Nuclear Pore Complex (NPC) Disassembly Pathway Diagram
Nuclear Pore Complex Pathway antibodies	Nuclear Pore Complex Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram

Organs/Tissues associated with mbo restriction enzyme

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>36 publications with mbo and Blood
Embryonic Tissue Antibodies	>5 publications with mbo and Embryonic Tissue
Ovary Antibodies	>3 publications with mbo and Ovary
Testis Antibodies	>2 publications with mbo and Testis
Whole Body Antibodies	>2 publications with mbo and Whole Body
Head Antibodies	>1 publications with mbo and Head
Fat Body Antibodies	>1 publications with mbo and Fat Body