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Bsppi Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638415

MBS638415 is a restriction enzyme targeting BspPI. Also known as BspPI (BinI). Host: Bacillus species d1-34. 5'-G G A T C (N)4^-3' More details

$450 / 100 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BspPI, Restriction Enzyme
Full Product Name
BspPI (BinI)
Host
Bacillus species d1-34
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Methylation Effects
BspPI does not cut GGm6ATC. Blocked by Dam methylation.
pACYC177
13
pACYC184
4
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda
58
Ligation/Recutting Assay
After 10-fold overdigestion (2u/ug DNA x 5 hours) with BspPI, approximately 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40u of T4 DNA ligase/1ug of fragments at a 5'-termini concentration of 0.2uM. More than 50% of these can be recut.
M13mp18/19
4
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with BspPI.
pBR322
12
PhiX174
0
pTZ19R/U
10
pUC18/19
10
pUC57
10
Stability During Prolonged Incubation
A minimum of 0.5units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 55 degree C.
Storage Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Enzyme is inactivated by incubation at 80 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dam-in 1 hour at 55 degree C in 50ul of assay buffer.

Product Categories/Family for BspPI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of BspPI restriction enzyme

Restriction Enzyme – Cat# MBS638415

Other Notes

Small volumes of BspPI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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