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Pvu I Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638420

MBS638420 is a restriction enzyme targeting Pvu I. 5'-C G A T^C G-3' More details

$515 / 300 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Pvu I, Restriction Enzyme
Full Product Name
Pvu I
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used.
Digestion of Agarose-embedded DNA
A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends
Bsh1285I, SgfI, PacI
Methylation Effects
PvuI does not cut CGATm5CG. Blocked by CG methylation.
pACYC177
2
pACYC184
0
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda
3
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with PvuI, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.03µM. More than 95% of these can be recut.
M13mp18/19
1
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with PvuI.
pBR322
1
PhiX174
0
pTZ19R/U
2
pUC18/19
2
pUC57
2
Stability During Prolonged Incubation
A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer
10mM Tris-HCl (pH 7.5 at 25 degree C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.02% Triton X-100, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Only small amounts of enzyme (up to 10 units) can be inactivated by incubation at 80 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-CpoI fragments in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for Pvu I restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of Pvu I restriction enzyme

Restriction Enzyme – Cat# MBS638420

Other Notes

Small volumes of Pvu I restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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