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Bfmi Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638423

MBS638423 is a restriction enzyme targeting BfmI. Also known as BfmI (SfeI). Host: Bacillus firmus S8-336. 5'-C^T Pu Py A G-3' More details

$490 / 200 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BfmI, Restriction Enzyme
Full Product Name
BfmI (SfeI)
Host
Bacillus firmus S8-336
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Compatible Ends
C^TGCAG-Alw44I
pACYC177
1
pACYC184
1
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda
38
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with BfmI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.5uM. More than 95% of these can be recut.
M13mp18/19
7
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with BfmI (see Star Activity).
pBR322
4
PhiX174
6
pTZ19R/U
6
pUC18/19
4
pUC57
4
Stability During Prolonged Incubation
A minimum of 1.0unit of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer
10mM Tris-HCl (pH 7.5 at 25 degree C), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for BfmI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of BfmI restriction enzyme

Restriction Enzyme – Cat# MBS638423

Other Notes

Small volumes of BfmI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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