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Bsp119i Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638427

MBS638427 is a restriction enzyme targeting Bsp119I. Also known as Bsp119I (AsuII). Host: Bacillus species RFL119. More details

$485 / 2500 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Bsp119I, Restriction Enzyme
Full Product Name
Bsp119I (AsuII)
Sequence
5'-T T^C G A A-3'
3' -A A G C^T T-5'
Host
Bacillus species RFL119
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Digestion of Agarose-embedded DNA
A minimum of 5units of Bsp119I is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Methylation Effects
Bsp119I does not cut TTm5CGAA. Blocked by CG methylation.
Supplied With
R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37ºC.
Number of Recognition Sites in DNA
Lambda: 7
PhiX174, M13mp18/19, pBR322, pUC18/19, pUC57, pTZ19R/U: 0
Stability During Prolonged Incubation
A minimum of 0.1units of Bsp119I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Thermal Inactivation
Bsp119I is inactivated by incubation at 80 degree C for 20min.
Unit Definition
One unit is defined as the amount of Bsp119I required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for Bsp119I restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of Bsp119I restriction enzyme

Restriction Enzyme – Cat# MBS638427

Other Notes

Small volumes of Bsp119I restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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