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Nmuci Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638438

MBS638438 is a restriction enzyme targeting NmuCI. Also known as NmuCI (Tsp45I). C 5'-^G T G A C-3' More details

$515 / 200 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NmuCI, Restriction Enzyme
Full Product Name
NmuCI (Tsp45I)
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
10mM Tris-HCl (pH 7.5 at 25 degree C), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used.
Compatible Ends
Eco91I (G^GTCACC) MaeIII (^GTGAC).
Methylation Effects
GT(C/G)m5C is cleaved by NmuCI at a significantly slower rate. Overlapping CG methylation may influence DNA cleavage.
pACYC177
5
pACYC184
7
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda
81
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with NmuCI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.8uM. More than 95% of these can be recut.
M13mp18/19
8
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with NmuCI.
pBR322
9
PhiX174
8
pTZ19R/U
4
pUC18/19
4
pUC57
4
Stability During Prolonged Incubation
A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for NmuCI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of NmuCI restriction enzyme

Restriction Enzyme – Cat# MBS638438

Other Notes

Small volumes of NmuCI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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