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Mbii Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638452

MBS638452 is a restriction enzyme targeting MbiI. Also known as MbiI (BsrBI). Host: Moraxella bovis Fr 1-022. 5'-G A G^C G G-3' More details

$530 / 1000 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MbiI, Restriction Enzyme
Full Product Name
MbiI (BsrBI)
Host
Moraxella bovis Fr 1-022
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Digestion of Agarose-embedded DNA
A minimum of 5 units of MbiI is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Supplied With
R1625: Restriction MbiI Buffer A, 10X
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda
17 pUC57: 3
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with MbiI, approximately 80% of the DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. Approximately 50% of these can be recut. DNA fragments, obtained after cleavage with MbiI (due to its asymmetric recognition sequence), may join each other in 3 different ways and in only one case is the resulting sequence recognized by MbiI. Other two sequences resulting from ligation of MbiI DNA fragments may be cleavaged with Cfr42I (SacII) and Ecl136II (SacI).
M13mp18/19
4 pUC18/19: 3
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with MbiI (see Star Activity).
pBR322
2
PhiX174
1 pTZ19R/U: 4
Stability During Prolonged Incubation
A minimum of 0.2 units of MbiI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer
10mM Tris-HCl, pH 7.4, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
MbiI is inactivated by incubation at 65 degree C for 20min.
Unit Definition
One unit is defined as the amount of MbiI required to digest 1ug of Lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for MbiI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of MbiI restriction enzyme

Restriction Enzyme – Cat# MBS638452

Other Notes

Small volumes of MbiI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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