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Alw21i Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638454

MBS638454 is a restriction enzyme targeting Alw21I. Also known as Alw21I (HgiAI). Host: Acinetobacter lwoffi RFL21. 5' - G W G C W^C - 3' More details

$410 / 500 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alw21I, Restriction Enzyme
Full Product Name
Alw21I (HgiAI)
Host
Acinetobacter lwoffi RFL21
Form/Format
Supplied as a liquid in 10mM Tris-HCl pH 7.5, 300mM sodium chloride, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplied With
R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA pH 7.9 at 37 degree C when diluted to 1X. Note: 1X conc. yields 20-50% enyzme activity. 2X conc. yields 50-100% enzyme activity.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Alw21I for 4 hours.
Lambda
28
M13mp18/19
3
pBR322
8
PhiX174
3
pTZ19R/U
4
pUC18/19
5
pUC57
5
Unit Definition
One unit is defined as the amount of Alw21I required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for Alw21I restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of Alw21I restriction enzyme

Restriction Enzyme – Cat# MBS638454

Other Notes

Small volumes of Alw21I restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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