Bpii Restriction Enzyme

Name: Restriction Enzyme; Bpii; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS638457
Price: 465 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638457

MBS638457 is a restriction enzyme targeting BpiI. Also known as BpiI (BbvII). 5'-G A A G A C (N)2v-3'3'-C T T C T G (N)6^-5' More details

$465 / 200 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BpiI, Restriction Enzyme

Full Product Name

BpiI (BbvII)

Purity/Purification

Molecular Biology Grade

Concentration

lOu/ul (lot specific)

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source

Bacillus pumillus Sw 4-3

Digestion of Agarose-embedded DNA

A minimum of 5units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.

Methylation Effects

Dam, Dcm, EcoKl: never overlaps - no effect
CpG may overlap - no effect
EcoBl: may overlap-effect not determined

Overdigestion Assay

No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BpiI.

Blue/White Cloning Assay

The B/W assay was relpaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test.

Labeled Oligonucleotide (LO) Assay

No detectable degradation of single-stranded or double-stranded labeled oligonucleotide occurred duringincubation with 10 units of Bpil for 4 hours.

Ligation/Recutting Assay

The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.

Number of Recognition Sites in DNA

Lambda: 24
PhiX174: 3
pBR322: 3
pUC57: 0
pUC18/19: 0
pTZ19R/U: 0
M13mp18/19: 0

Stability During Prolonged Incubation

A minimum of 0.3units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.

Diluent Buffer

10mM Tris-HCl, pH 7.4 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.

Thermal Inactivation

Bpil is inactivated by incubation at 65 degree C for 20 minutes.

Unit Definition

One unit is defined as the amount of BpiI required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of reaction buffer.

Supplied With

MBS638383: Restriction Enzyme Buffer A, 10X, Dilute to 1X. 1X buffer compostion is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
MBS638414: Restriction Enzyme Buffer C, 10X, Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2, 50mM sodium chloride, 0.1mg/ml BSA.

Protocol for Digestion

Water nuclease free: 16u
MBS638414: 2ul
DNA(0.5-1ug/ul): 1 ul
MBS638457: 0.5-2UL
Mix gently and spin down for a few seconds. Incubate at 37 degree C for 1-16 hours. The digestation reaction may be either scaled up or down.

Protocol for Digestion of PCR products Directly Amplification

PCR Reaction Mixture: 10ul (about 0.1-0.5ug of DNA)
Water, nuclease free: 18ul
MBS638414: 2ul
MBS638457: 1-2ul
Mix gently and spin down for a few seconds. Incubate at 37 degree C for 1-16 hours.

Product Categories/Family for BpiI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Other Notes

Small volumes of BpiI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BpiI. It may not necessarily be applicable to this product.

NCBI GI #

408407672

NCBI Accession #

P0DJJ9.1

NCBI Official Full Name

Basic phospholipase A2 BP-II

Protein Family

Basic phospholipase

UniProt Comments for BpiI

Snake venom phospholipase A2 (PLA2) that shows anticoagulant activities, strong myolytic activity, infiltration of polymorphonuclear cells, and edema in stromal tissues. Induces cell death of Jurkat cells in a concentration dependent manner. Shows a low phospholipase A2 activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.