Muni Restriction Enzyme

Name: Restriction Enzyme; Muni; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS638458
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638458

MBS638458 is a restriction enzyme targeting MunI. Also known as MunI (MfeI). 5'-C^A A T T G-3' More details

$450 / 300 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MunI, Restriction Enzyme

Full Product Name

MunI (MfeI)

Purity/Purification

Molecular Biology Grade

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Dilution Buffer

10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.

Digestion of Agarose-embedded DNA

A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.

Supplied With

R1625-Restriction Enzyme Buffer A, 10X:
Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37 degree C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.

Blue/White Cloning Assay

The mix of pUC57/HindIII, pUC57/PstI and pUC57/Eco32I digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation < 1% of white colonies were detected.

Labeled Oligonucleotide (LO) Assay

No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of enzyme for 4 hours.

Lambda

Ligation/Recutting Assay

After 50-fold overdigestion (3u/ug DNA x 17 hours) with MunI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.07uM. More than 95% of these can be recut.

M13mp18/19

Overdigestion Assay

No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with MunI.

pBR322

PhiX174

pTZ19R/U

pUC18/19

pUC57

Stability During Prolonged Incubation

A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.

Storage Buffer

10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.

Thermal Inactivation

Enzyme is inactivated by incubation at 65 degree C for 20min.

Unit Definition

One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for MunI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Other Notes

Small volumes of MunI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MunI. It may not necessarily be applicable to this product.

NCBI GI #

1171057

NCBI Accession #

P43641.1

NCBI Official Full Name

Modification methylase MunI

Protein Family

Modification methylase

UniProt Comments for MunI

This methylase recognizes the double-stranded sequence CAATTG, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the MunI endonuclease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.