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Bseli Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638461

MBS638461 is a restriction enzyme targeting BseLI. Also known as BseLI (BslI). 5'-C C N N N N N^N N G G-3' More details

$450 / 500 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BseLI, Restriction Enzyme
Full Product Name
BseLI (BslI)
Purity/Purification
Molecular Biology Grade
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Dilution Buffer
For short term storage (3-4 weeks)- dilute with 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Supplied With
R1625: Restriction Enzyme Buffer A, 10X
Lambda
176
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with BseLI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.6uM. More than 95% of these can be recut.
M13mp18/19
17
Note
BseLI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam, dcm strain such as GM2163.
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BseLI.
pBR322
20
pTZ19R/U
7
Storage Buffer
For long term storage: 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Unit Definition
One unit is defined as the amount of BseLI required to digest 1ug of lambda DNA dcm-in 1 hour at 55 degree C in 50ul of assay buffer.

Product Categories/Family for BseLI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of BseLI restriction enzyme

Restriction Enzyme – Cat# MBS638461

Other Notes

Small volumes of BseLI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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