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Mssi Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638484

MBS638484 is a restriction enzyme targeting MssI. Also known as MssI (PmeI). Host: Methylobacterium species Dd 5-732. 5'-G T T T^A A A C-3' More details

$410 / 250 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MssI, Restriction Enzyme
Full Product Name
MssI (PmeI)
Host
Methylobacterium species Dd 5-732
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied With
R1625-Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37 degree C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of Mssl for 4 hours.
Lambda
2
M13mp18/19
0
pBR322
0
PhiX174
0
pTZ19R/U
0
pUC18/19
0
pUC57
0
Storage Buffer
10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Unit Definition
One unit is defined as the amount of Mssl required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for MssI restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of MssI restriction enzyme

Restriction Enzyme – Cat# MBS638484

Other Notes

Small volumes of MssI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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