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Alw44i Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638487

MBS638487 is a restriction enzyme targeting Alw44I. Also known as Alw44I (ApaLI). 5'-G^T G C A C-3' More details

$435 / 1000 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alw44I, Restriction Enzyme
Full Product Name
Alw44I (ApaLI)
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Supplied With
R1625-Restriction Enzyme Buffer A (double digests): 1X Buffer composition-Supplied as a liquid containing 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Incubate at 37 degree C.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide occurred during incubation with 10 units of Alw44l for 4 hours.
Lambda
4
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with Alw44I more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.03uM. More than 95% of these can be recut.
pBR322
3
PhiX174
1
pTZ19R/U
2
pUC18/19
3
pUC57
3
Storage Buffer
Supplied as a liquid in 10mM Tris-HCl pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Thermal Inactivation
Enzyme is inactivated by incubation at 65 degree C for 20 minutes.
Unit Definition
One unit is defined as the amount of Alw44l required to digest 1ug of lambda DNA-Smal fragments in 1 hour at 37ºC in 50ul of assay buffer.

Product Categories/Family for Alw44I restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of Alw44I restriction enzyme

Restriction Enzyme – Cat# MBS638487

Other Notes

Small volumes of Alw44I restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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