Bpl I (bpl) Restriction Enzyme

Name: bpl Restriction Enzyme; Bpl I; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS638493
Price: 500 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638493

BplI requires only Mg2+ for its activity, but is stimulated by S-adenosylmethionine. 0.05mM S-adenosylmethionine gives more than a 100-fold increase of BplI activity. The cleavage of DNA by BplI is never complete.5'-^ 8(N) G A G (N)5 C T C (N)13^-3'3'-^13(N)C… More details

$500 / 100 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Bpl I, Restriction Enzyme

Full Product Name

Bpl I

Product Gene Name

bpl restriction enzyme

Host

Bacilus pumilus

Purity/Purification

Molecular Biology Grade

Concentration

5 units/uL (lot specific)

Preparation and Storage

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chromosome Location

NC_003552.1 (792605..793582)

Storage Buffer

Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.

Unit Definition

One unit is defined as the amount of Bpl I required to digest 1ug of lambda DNA-Xhol in 1 hour at 37°C in 50ul of assay buffer (a maximal cleavage level is achieved at which no change in the fragmentation pattern is observed with further increase of enzyme).

Buffer Supplied

MBS638383 Restndion Enzyme Buffer A, lOX: 33mM Tns-acetate, pH 7.9, 10mM magnesium acetate,66mM potassium acetate, 0.1 mg/ml bSA
SAM: 0.05mM S-adenosylmethionine

Stability during Prolonged Incubation

A minimum of 0.3 units of Bpl l is required for complete digestion of 1 ug of DNA in 16 hours at 37°C.

Thermal Inactivation

Bpl l is inactivated by incubation at 65°C for 20 mins.

Methylation Effect

DAM, DCM, EcoKl, EcoBl: never overlaps no effect.
CpG: may overlap- no effect.

Number of Recognition Sites in DNA

Lambda: 1
PhiX174, M13mp1Sj19, pBR322,
pUC1S/19, pUCS7,
pTZ19R/U: 0

Digestion of Agarose-embedded DNA

A minimum of 10 units of Bpi l is required for complete digestion of 1 ug of agarose-embedded lambda DNA in 16 hours.

Overdigestion Assay

No detectable change in the specific fragmentation pattern is observed after 16D-fold overdigestion (10u/ug lambda DNA x 16 hours) with Bpll.

Ligation/Recutting Assay

The ligation and recreavage assay was replaced with LO test after validating experiments showed LO test ability10 trace nuclease and phosp,hatase activities with sensitivity that is higher thab L/R by a factor of 100.

Labeled Oligonucleotide (La) Assay

No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Bpl I for 4 hours.

Blue/White Cloning Assay

The B/W assay was replaced with LO test after validating experiments showed LO test ability to aetect nuclease and phosphatase activities with sensitivity that equals to that of B/W test.

Product Categories/Family for bpl restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Other Notes

Small volumes of bpl restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for bpl purchase

MBS638493 is a restriction enzyme targeting bpl. Also known as Bpl I. Host: Bacilus pumilus.

Additional Details

NCBI/Uniprot data below describe general gene information for bpl. It may not necessarily be applicable to this product.

NCBI GI #

NCBI GeneID

NCBI Accession #

GenBank Nucleotide #

NCBI Official Full Name

biotin operon repressor/biotin-pyruvate carboxylase ligase

NCBI Official Symbol

bpl

NCBI Official Synonym Symbols

birA

NCBI Protein Information

biotin operon repressor/biotin-pyruvate carboxylase ligase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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