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NRF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6404571

MBS6404571 is an antibody targeting NRF1. Also known as NRF1 (Alpha palindromic-binding protein) (HRP). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$825 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
NRF1, Polyclonal Antibody
Full Product Name
NRF1 (Alpha palindromic-binding protein) (HRP)
Product Gene Name
anti-NRF1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human NRF1. Does not react with other proteins. Species Crossreactivity: mouse, rat
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
53,541 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (129251555..129396922). Location: 7q32
OMIM
600879
3D Structure
ModBase 3D Structure for Q16656
Immunogen
Synthetic peptide corresponding to aa272-288, QHGREDLLYAFEDQQTQ of human NRF1. Identical to the related rat and mouse sequences.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-NRF1 antibody

Antibodies; Binding Proteins
Applications Tested/Suitable for anti-NRF1 antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-NRF1 antibody
ICC: 0.5-1ug/ml
WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml. Boil paraffin sections in 10mM citrate buffer, pH 6.0, for 20 min is required.
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NRF1 antibody

Anti-NRF1 antibody, 144992, Western blot Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: MCF-7 Cell Lysate Lane 4: A549 Cell Lysate
Rabbit Anti-NRF1 Polyclonal Antibody – Cat# MBS6404571

Immunohistochemistry (IHC) of anti-NRF1 antibody

Anti-NRF1 antibody, 144992, Immunohistochemistry(P) IHC(P): Human Mammary Cancer Tissue
Rabbit Anti-NRF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6404571

Immunocytochemistry (ICC) of anti-NRF1 antibody

Anti-NRF1 antibody, 144992, Immunocytochemistry ICC: A549 Cell
Rabbit Anti-NRF1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS6404571

Immunohistochemistry (IHC) of anti-NRF1 antibody

Anti-NRF1 antibody, 144992, Immunohistochemistry(P) IHC(P): Rat Intestine Tissue
Rabbit Anti-NRF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6404571

Immunohistochemistry (IHC) of anti-NRF1 antibody

Anti-NRF1 antibody, 144992, Immunohistochemistry(P) IHC(P): Human Intestinal Cance Tissue
Rabbit Anti-NRF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6404571

Product References and Citations for anti-NRF1 antibody

1. Efiok, B. J. S., Chiorini, J. A., Safer, B. A key transcription factor for eukaryotic initiation factor-2-alpha is strongly homologous to developmental transcription factors and may link metabolic genes to cellular growth and development. J. Biol. Chem. 269: 18921-18930, 1994. 2. Gopalakrishnan, L., Scarpulla, R. C. Structure, expression, and chromosomal assignment of the human gene encoding nuclear respiratory factor 1. J. Biol. Chem. 270: 18019-18025, 1995. 3. Virbasius, J. V., Scarpulla, R. C. Activation of the human mitochondrial transcription factor A gene by nuclear respiratory factors: a potential regulatory link between nuclear and mitochondrial gene expression in organelle biogenesis. Proc. Nat. Acad. Sci. 91: 1309-1313, 1994.

Other Notes

Small volumes of anti-NRF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NRF1. It may not necessarily be applicable to this product.

NCBI GI #
93141039
NCBI GeneID
4899
NCBI Accession #
NP_001035199.1
UniProt Primary Accession #
Q16656
UniProt Secondary Accession #
Q15305
UniProt Related Accession #
Q16656
GenBank Nucleotide #
NM_001040110.1
NCBI Official Full Name
nuclear respiratory factor 1
NCBI Official Synonym Full Names
nuclear respiratory factor 1
NCBI Official Symbol
NRF1
NCBI Official Synonym Symbols
ALPHA-PAL
UniProt Protein Name
Nuclear respiratory factor 1
UniProt Synonym Protein Names
Alpha palindromic-binding protein
Protein Family
Nuclear respiratory factor
UniProt Gene Name
NRF1
UniProt Synonym Gene Names
NRF-1; Alpha-pal
UniProt Entry Name
NRF1_HUMAN
NCBI Protein Information
nuclear respiratory factor 1; NRF-1; alpha palindromic-binding protein

NCBI Summary for NRF1

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, Jul 2008]
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UniProt Comments for NRF1

NRF1: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Belongs to the NRF1/Ewg family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: nucleoplasm; cytoplasm

Biological Process: regulation of transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; organ regeneration; generation of precursor metabolites and energy; transcription, DNA-dependent; response to folic acid; organelle organization and biogenesis; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; response to lipopolysaccharide; cellular lipid metabolic process; response to electrical stimulus; response to estradiol stimulus
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Research Articles on NRF1

1. NRF-1 regulates Atp1a1 and Atp1b1 and are important in mediating the energy generation and neuronal activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NRF1 antibody

 Reference Product  PubMed Publications
 TFAM antibody  >72 publications with NRF1 and TFAM
 PPARGC1A antibody  >40 publications with NRF1 and PPARGC1A
 MAFF antibody  >4 publications with NRF1 and MAFF

Pathways associated with anti-NRF1 antibody

 Products by Pathway  Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Mitochondrial Gene Expression Pathway antibodies  Mitochondrial Gene Expression Pathway Diagram
 PPARA Activates Gene Expression Pathway antibodies  PPARA Activates Gene Expression Pathway Diagram
 Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies  Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram

Diseases associated with anti-NRF1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >22 publications with NRF1 and Nervous System Diseases
 Insulin Resistance Antibodies  >19 publications with NRF1 and Insulin Resistance
 Diabetes Mellitus Antibodies  >17 publications with NRF1 and Diabetes Mellitus
 Liver Diseases Antibodies  >15 publications with NRF1 and Liver Diseases
 Inflammation Antibodies  >14 publications with NRF1 and Inflammation
 Necrosis Antibodies  >13 publications with NRF1 and Necrosis
 Cardiovascular Diseases Antibodies  >12 publications with NRF1 and Cardiovascular Diseases
 Drug Toxicity Antibodies  >12 publications with NRF1 and Drug Toxicity
 Fatty Liver Antibodies  >11 publications with NRF1 and Fatty Liver
 Disease Models, Animal Antibodies  >10 publications with NRF1 and Disease Models, Animal

Organs/Tissues associated with anti-NRF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >39 publications with NRF1 and Liver
 Blood Antibodies  >24 publications with NRF1 and Blood
 Brain Antibodies  >22 publications with NRF1 and Brain
 Heart Antibodies  >21 publications with NRF1 and Heart
 Lung Antibodies  >12 publications with NRF1 and Lung
 Kidney Antibodies  >11 publications with NRF1 and Kidney
 Skin Antibodies  >11 publications with NRF1 and Skin
 Embryonic Tissue Antibodies  >8 publications with NRF1 and Embryonic Tissue
 Nerve Antibodies  >8 publications with NRF1 and Nerve
 Eye Antibodies  >7 publications with NRF1 and Eye
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