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HNMT Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641130

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HNMT, Monoclonal Antibody
Full Product Name
HNMT (Histamine N-methyltransferase, HMT)
Product Gene Name
Product Synonym Names
Anti -HNMT (Histamine N-methyltransferase, HMT)
Immunogen Sequence
KKYGSRFPQD DLCQYITSDD LTQMLDNLGL KYECYDLLST MDISDCFIDG NENGDLLWDF LTETCNFNAT APPDLRAELG KDLQEPEFSA KKEGKVLFNN TLSFIVIEA
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3G12
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human HNMT.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
33,295 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (138721808..138773934). Location: 2q22.1
OMIM
605238
3D Structure
ModBase 3D Structure for P50135
Immunogen
Partial recombinant corresponding to aa184-292 from human HNMT (NP_008826) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-HNMT antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-HNMT antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-HNMT antibody

Western Blot detection against Immunogen (37.73D)
Mouse Anti-HNMT Monoclonal Antibody – Cat# MBS641130

Western Blot (WB) of anti-HNMT antibody

HNMT monoclonal antibody Western Blot analysis of HNMT expression in HeLa.
Mouse Anti-HNMT Monoclonal Antibody, Western Blot (WB) – Cat# MBS641130

Testing Data of anti-HNMT antibody

Detection limit for recombinant GST tagged HNMT is ~0.1ng/ml as a capture antibody.
Mouse Anti-HNMT Monoclonal Antibody, Testing Data – Cat# MBS641130

Other Notes

Small volumes of anti-HNMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HNMT purchase

MBS641130 is an antibody targeting HNMT. Also known as HNMT (Histamine N-methyltransferase, HMT). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HNMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histamine N-methyltransferase isoform 3
NCBI Official Synonym Full Names
histamine N-methyltransferase
NCBI Official Symbol
HNMT
NCBI Official Synonym Symbols
HMT; HNMT-S1; HNMT-S2
UniProt Protein Name
Histamine N-methyltransferase
UniProt Gene Name
HNMT
UniProt Synonym Gene Names
HMT
UniProt Entry Name
HNMT_HUMAN
NCBI Protein Information
histamine N-methyltransferase

UniProt Comments for HNMT

HNMT: Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine. Belongs to the methyltransferase superfamily. HNMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.8; Amino Acid Metabolism - histidine; Methyltransferase

Chromosomal Location of Human Ortholog: 2q22.1

Cellular Component: nucleoplasm; neuron projection; cytoplasm

Molecular Function: histamine N-methyltransferase activity

Biological Process: methylation; hyperosmotic response; response to tumor cell; response to glucocorticoid stimulus; respiratory gaseous exchange; brain development; response to cocaine; response to amine stimulus

Disease: Asthma, Susceptibility To
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HNMT antibody

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