Sheep Anti-UBASH3B Polyclonal Antibody – Cat# MBS641201

Detection of Human and Rat UBASH3B/STS1 by Western Blot. Western blot shows lysates of HCT116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, MOLT4 human acute lymphoblastic leukemia cell line, U118MG human glioblastoma/astrocytoma cell line, U2OS human osteosarcoma cell line, and Y3Ag rat myeloid cell line. PVDF Membrane was probed with 2ug/ml of Human/Rat UBASH3B/STS1 Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated Sheep IgG Secondary Antibody. A specific band was detected for UBASH3B/STS1 at approximately 72kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

UBASH3B Antibody – Sheep Polyclonal

Name: UBASH3B Antibody; Polyclonal; Sheep; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS641201
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641201

UBASH3B is a cytosolic 72-74kD member of the TulA (T cell ubiquitin ligand) family of proteins. It is widely expressed and appears to perform multiple functions. As a histidine phosphatase, it dephosphorylates Syk and Syk-related molecules, thus dampening… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

UBASH3B, Polyclonal Antibody

Full Product Name

UBASH3B (Ubiquitin-associated and SH3 Domain-containing B, Cbl-interacting Protein 4, CLIP4, Suppressor of T Cell Receptor Signaling 2, STS 2, STS2, STS-2, T Cell Ubiquitin Ligand, TULA)

Product Gene Name

anti-UBASH3B antibody

Product Synonym Names

Anti -UBASH3B (Ubiquitin-associated and SH3 Domain-containing B, Cbl-interacting Protein 4, CLIP4, Suppressor of T Cell Receptor Signaling 2, STS 2, STS2, STS-2, T Cell Ubiquitin Ligand, TULA)

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Species Reactivity

Human, Rat

Form/Format

Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 200ug/ml.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human UBASH3B. Species Crossreactivity: rat.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

72,696 Da

Chromosome Location

Chromosome: 11; NC_000011.9 (122526398..122685187). Location: 11q24.1

OMIM

609201

3D Structure

ModBase 3D Structure for Q8TF42

Immunogen

Recombinant corresponding to aa48-253 from human UBASH3B/STS1 expressed in E. coli (Q8TF42).

Product Categories/Family for anti-UBASH3B antibody

Antibodies; Abs to Ubiquitin

Applications Tested/Suitable for anti-UBASH3B antibody

Western Blot (WB)

Application Notes for anti-UBASH3B antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 2ug/ml

Other Notes

Small volumes of anti-UBASH3B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBASH3B purchase

MBS641201 is an antibody targeting UBASH3B. Also known as UBASH3B (Ubiquitin-associated and SH3 Domain-containing B, Cbl-interacting Protein 4, CLIP4, Suppressor of T Cell Receptor Signaling 2, STS 2, STS2, STS-2, T Cell Ubiquitin Ligand, TULA). Reactive in Human, Rat. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for UBASH3B. It may not necessarily be applicable to this product.

NCBI GI #

24497612

NCBI GeneID

84959

NCBI Accession #

NP_116262.2

UniProt Primary Accession #

Q8TF42

UniProt Secondary Accession #

Q53GT5; Q53GT8; Q8NBV7; Q96IG9; Q96NZ2

UniProt Related Accession #

Q8TF42

GenBank Nucleotide #

NM_032873.4

NCBI Official Full Name

ubiquitin-associated and SH3 domain-containing protein B

NCBI Official Synonym Full Names

ubiquitin associated and SH3 domain containing B

NCBI Official Symbol

UBASH3B

NCBI Official Synonym Symbols

p70; STS1; STS-1; TULA-2

UniProt Protein Name

Ubiquitin-associated and SH3 domain-containing protein B

UniProt Synonym Protein Names

Cbl-interacting protein p70; Suppressor of T-cell receptor signaling 1; STS-1; T-cell ubiquitin ligand 2; TULA-2; Tyrosine-protein phosphatase STS1/TULA2

Protein Family

Ubiquitin-associated and SH3 domain-containing protein

UniProt Gene Name

UBASH3B

UniProt Synonym Gene Names

KIAA1959; STS1; STS-1; TULA-2

UniProt Entry Name

UBS3B_HUMAN

NCBI Protein Information

ubiquitin-associated and SH3 domain-containing protein B; T-cell ubiquitin ligand 2; cbl-interacting protein p70; Cbl-interacting protein Sts-1; nm23-phosphorylated unknown substrate; tyrosine-protein phosphatase STS1/TULA2; suppressor of T-cell receptor signaling 1; SH3 domain-containing 70 kDa protein, suppressor of T-cell receptor signaling 1, nm23-phosphorylated unknown substrate

NCBI Summary for UBASH3B

This gene encodes a protein that contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. The encoded protein was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor. [provided by RefSeq, Jul 2008]

UniProt Comments for UBASH3B

STS-1: Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.

Protein type: Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; protein tyrosine phosphatase activity

Biological Process: negative regulation of protein kinase activity; regulation of release of sequestered calcium ion into cytosol

Research Articles on UBASH3B

1. UBASH3B is a functional target of anti-invasive microRNA200a that is down-regulated in triple negative breast cancer. Importantly, the oncogenic potential of UBASH3B is dependent on its tyrosine phosphatase activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBASH3B antibody

Reference Product	PubMed Publications
SYK antibody	>1 publications with UBASH3B and SYK
GRB2 antibody	>1 publications with UBASH3B and GRB2
EGFR antibody	>1 publications with UBASH3B and EGFR
CBL antibody	>1 publications with UBASH3B and CBL

Diseases associated with anti-UBASH3B antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>3 publications with UBASH3B and Cardiovascular Diseases
Neoplasm Metastasis Antibodies	>1 publications with UBASH3B and Neoplasm Metastasis
Neoplasms Antibodies	>1 publications with UBASH3B and Neoplasms
Breast Neoplasms Antibodies	>1 publications with UBASH3B and Breast Neoplasms

Organs/Tissues associated with anti-UBASH3B antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3 publications with UBASH3B and Blood
Skin Antibodies	>2 publications with UBASH3B and Skin