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DPH1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641216

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of… More details

$880 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DPH1, Monoclonal Antibody
Full Product Name
DPH1 (Diphthamide Biosynthesis Protein 1, DPH2-like 1, DPH2L1, DPH2L, FLJ33211, Ovarian Cancer Associated Gene 1, OVCA1)
Product Gene Name
Product Synonym Names
Anti -DPH1 (Diphthamide Biosynthesis Protein 1, DPH2-like 1, DPH2L1, DPH2L, FLJ33211, Ovarian Cancer Associated Gene 1, OVCA1)
Immunogen Sequence
ILGCTSPRLS KEVEAVVYLG DGRFHLESVM IANPNVPAYR YDPYSKVLSR EHYDHQRMQA ARQEAIATAR SAKSWGLILG TLGRQGSPKI LEHLESRLRA LGLSFVRLL
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2C5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human DPH1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
48,805 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (1933431..1947183). Location: 17p13.3
OMIM
603527
3D Structure
ModBase 3D Structure for Q9BZG8
Immunogen
Partial recombinant corresponding to aa216-324 from DPH1 (NP_001374) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-DPH1 antibody

Applications Tested/Suitable for anti-DPH1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-DPH1 antibody
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 10ug/ml

Western Blot (WB) of anti-DPH1 antibody

DPH1 monoclonal antibody. Western Blot analysis of DPH1 expression in HeLa.
Mouse Anti-DPH1 Monoclonal Antibody – Cat# MBS641216

Immunofluorescence (IF) of anti-DPH1 antibody

Immunofluorescence of monoclonal antibody to DPH1 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-DPH1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS641216

Other Notes

Small volumes of anti-DPH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DPH1 purchase

MBS641216 is an antibody targeting DPH1. Also known as DPH1 (Diphthamide Biosynthesis Protein 1, DPH2-like 1, DPH2L1, DPH2L, FLJ33211, Ovarian Cancer Associated Gene 1, OVCA1). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DPH1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DPH1 protein
NCBI Official Synonym Full Names
diphthamide biosynthesis 1
NCBI Official Symbol
DPH1
NCBI Official Synonym Symbols
DPH2L; OVCA1; DPH2L1
UniProt Protein Name
Diphthamide biosynthesis protein 1
UniProt Synonym Protein Names
DPH1 homolog; HsDph1; Diphthamide biosynthesis protein 2 homolog-like 1; DPH-like 1; DPH2-like 1; Diphthamide biosynthesis protein 2-like; Ovarian cancer-associated gene 1 protein
UniProt Gene Name
DPH1
UniProt Synonym Gene Names
DPH2L; DPH2L1; OVCA1; HsDph1; DPH-like 1; DPH2-like 1
UniProt Entry Name
DPH1_HUMAN
NCBI Protein Information
diphthamide biosynthesis protein 1; DPH-like 1; DPH2-like 1; DPH1 homolog; ovarian tumor suppressor candidate 1; ovarian cancer-associated gene 1 protein; candidate tumor suppressor in ovarian cancer 1; diphthamide biosynthesis protein 2 homolog-like 1; diptheria toxin resistance protein required for diphthamide biosynthesis-like 1; diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces)-like 1

UniProt Comments for DPH1

DPH1: Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers. Plays a role in embryonic growth, organogenesis and postnatal survival. Belongs to the DPH1/DPH2 family. DPH1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: nucleus; cytosol

Biological Process: cell proliferation; cellular protein metabolic process; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; post-translational protein modification
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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