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msh2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641269

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MSH2, Polyclonal Antibody
Full Product Name
MSH2, ID (MSH2, DNA mismatch repair protein Msh2, MutS protein homolog 2)
Product Gene Name
Product Synonym Names
Anti -MSH2, ID (MSH2, DNA mismatch repair protein Msh2, MutS protein homolog 2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
109,739 Da
3D Structure
ModBase 3D Structure for O74773
Immunogen
MSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644-673 amino acids from the Central region of human MSH2.
Applications Tested/Suitable for anti-msh2 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-msh2 antibody
Suitable for use in Western Blot, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-msh2 antibody

Western Blot analysis in SW480, U251 cell line lysates (35ug/lane) using MBS641269. This demonstrates that MBS641269 detected the MSH2 protein (arrow).
Rabbit Anti-msh2 Polyclonal Antibody – Cat# MBS641269

Immunofluorescence (IF) of anti-msh2 antibody

Confocal immunofluorescent analysis with Hela cells using MBS641269 followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-msh2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS641269

Flow Cytometry (FC/FACS) of anti-msh2 antibody

Flow Cytometric analysis of Hela cells using MBS641269 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-msh2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS641269

Other Notes

Small volumes of anti-msh2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for msh2 purchase

MBS641269 is an antibody targeting msh2. Also known as MSH2, ID (MSH2, DNA mismatch repair protein Msh2, MutS protein homolog 2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for msh2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
Msh2
UniProt Protein Name
DNA mismatch repair protein msh2
UniProt Gene Name
msh2
UniProt Entry Name
MSH2_SCHPO

UniProt Comments for msh2

Function: Involved in post-replicative DNA-mismatch repair. Binds to mismatch-containing DNA. Required for correct termination of copy synthesis during mating-type switching. Also required for proper chromosome organization during meiosis.

Subunit structure: Heterodimer of msh2 and msh6

By similarity.

Subcellular location: Nucleus

By similarity.

Sequence similarities: Belongs to the DNA mismatch repair MutS family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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