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TSSK4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641308

This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TSSK4, Polyclonal Antibody
Full Product Name
TSSK4, CT (TSSK4, C14orf20, STK22E, TSSK5, Testis-specific serine/threonine-protein kinase 4, Serine/threonine-protein kinase 22E)
Product Gene Name
Product Synonym Names
Anti -TSSK4, CT (TSSK4, C14orf20, STK22E, TSSK5, Testis-specific serine/threonine-protein kinase 4, Serine/threonine-protein kinase 22E)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
37,454 Da
Chromosome Location
Chromosome: 14; NC_000014.8 (24674753..24677568). Location: 14q12
OMIM
610711
3D Structure
ModBase 3D Structure for Q6SA08
Immunogen
TSSK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-262 amino acids from the C-terminal region of human TSSK4.
Applications Tested/Suitable for anti-TSSK4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-TSSK4 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-50

Western Blot (WB) of anti-TSSK4 antibody

Western blot analysis of TSSK4 (arrow) using rabbit polyclonal TSSK4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TSSK4 gene.
Rabbit Anti-TSSK4 Polyclonal Antibody – Cat# MBS641308

Immunohistochemistry (IHC) of anti-TSSK4 antibody

TSSK4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSSK4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-TSSK4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS641308

Immunofluorescence (IF) of anti-TSSK4 antibody

Confocal immunofluorescent analysis of TSSK4 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-TSSK4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS641308

Other Notes

Small volumes of anti-TSSK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TSSK4 purchase

MBS641308 is an antibody targeting TSSK4. Also known as TSSK4, CT (TSSK4, C14orf20, STK22E, TSSK5, Testis-specific serine/threonine-protein kinase 4, Serine/threonine-protein kinase 22E). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TSSK4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
testis-specific serine/threonine-protein kinase 4 isoform 1
NCBI Official Synonym Full Names
testis-specific serine kinase 4
NCBI Official Symbol
TSSK4
NCBI Official Synonym Symbols
TSK4; TSK-4; TSSK5; STK22E; C14orf20
UniProt Protein Name
Testis-specific serine/threonine-protein kinase 4
UniProt Synonym Protein Names
Serine/threonine-protein kinase 22E
UniProt Gene Name
TSSK4
UniProt Synonym Gene Names
C14orf20; STK22E; TSSK5; TSK-4; TSSK-4
UniProt Entry Name
TSSK4_HUMAN
NCBI Protein Information
testis-specific serine/threonine-protein kinase 4; serine/threonine kinase 22E; serine/threonine-protein kinase 22E

UniProt Comments for TSSK4

TSSK4: May be involved in a signaling pathway during male germ cell development or mature sperm function. Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CAMK group; TSSK family

Chromosomal Location of Human Ortholog: 14q12

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding

Biological Process: multicellular organismal development; activation of CREB transcription factor; regulation of signal transduction; spermatogenesis; cell differentiation; protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TSSK4 antibody

Organs/Tissues associated with anti-TSSK4 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >6 publications with TSSK4 and Testis
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