Mouse Anti-KYNU Monoclonal Antibody – Cat# MBS641672

Western Blot|Detection of Human Kynureninase by Western Blot. Western blot shows lysates of human liver tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 2ug/ml of Mouse Anti Human Kynureninase Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for Kynureninase at approximately 52kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Kynureninase (KYNU) Antibody – Mouse Monoclonal

Name: KYNU Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS641672
Price: 995 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641672

Kynureninase (KYNU) catalyzes the hydrolytic cleavage of the aa's L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3-hydroxyanthranilic acid and alanine. KYNU and other "kynurenine pathway" enzymes degrade dietary tryptophan in the… More details

$995 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Kynureninase, Monoclonal Antibody

Full Product Name

Kynureninase (L-kynurenine Hydrolase, KYNU)

Product Gene Name

anti-Kynu antibody

Product Synonym Names

Anti -Kynureninase (L-kynurenine Hydrolase, KYNU)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

12K123

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

Purity/Purification

Affinity Purified
Purified by Protein A/G affinity chromatography.

Specificity

Recognizes human Kynureninase. Species Crossreactivity: Recombinant mouse Kynureninase (10%).

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

52,470 Da

Chromosome Location

Chromosome: 3; NC_005102.3 (33622183..33770773). Location: 3q12

3D Structure

ModBase 3D Structure for P70712

Immunogen

Recombinant corresponding to aa1-465 from human Kynureninase expressed in Sf21 cell line.

Product Categories/Family for anti-Kynu antibody

Antibodies; Abs to Enzymes

Applications Tested/Suitable for anti-Kynu antibody

Western Blot (WB)

Application Notes for anti-Kynu antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 2ug/ml

Other Notes

Small volumes of anti-Kynu antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Kynu purchase

MBS641672 is an antibody targeting Kynu. Also known as Kynureninase (L-kynurenine Hydrolase, KYNU). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Kynu. It may not necessarily be applicable to this product.

NCBI GI #

16758776

NCBI GeneID

116682

NCBI Accession #

NP_446354.1

UniProt Primary Accession #

P70712

UniProt Secondary Accession #

Q68G25; Q7M0D0; Q9QW90

UniProt Related Accession #

P70712

GenBank Nucleotide #

NM_053902.2

NCBI Official Full Name

kynureninase

NCBI Official Synonym Full Names

kynureninase

NCBI Official Symbol

Kynu

UniProt Protein Name

Kynureninase

UniProt Synonym Protein Names

L-kynurenine hydrolase

Protein Family

Kynureninase

UniProt Gene Name

Kynu

UniProt Entry Name

KYNU_RAT

NCBI Protein Information

kynureninase; L-kynurenine hydrolase

NCBI Summary for Kynu

enzyme involved in the biosynthesis of NAD cofactors from tryptophan [RGD, Feb 2006]

UniProt Comments for Kynu

KYNU: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. Belongs to the kynureninase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Hydrolase; Amino Acid Metabolism - tryptophan; EC 3.7.1.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol

Molecular Function: protein homodimerization activity; kynureninase activity; pyridoxal phosphate binding

Biological Process: anthranilate metabolic process; tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine; quinolinate biosynthetic process; tryptophan catabolic process

Research Articles on Kynu

1. Kynureninase is a novel candidate gene for hypertension in spontaneously hypertensive rats.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Kynu antibody

Products by Pathway	Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Tryptophan Catabolism Pathway antibodies	Tryptophan Catabolism Pathway Diagram
Tryptophan Metabolism Pathway antibodies	Tryptophan Metabolism Pathway Diagram
Tryptophan Metabolism Pathway antibodies	Tryptophan Metabolism Pathway Diagram
Tryptophan Metabolism Pathway antibodies	Tryptophan Metabolism Pathway Diagram
Tryptophan Degradation III (eukaryotic) Pathway antibodies	Tryptophan Degradation III (eukaryotic) Pathway Diagram
Tryptophan Degradation XI (mammalian, Via Kynurenine) Pathway antibodies	Tryptophan Degradation XI (mammalian, Via Kynurenine) Pathway Diagram
Tryptophan Degradation To 2-amino-3-carboxymuconate Semialdehyde Pathway antibodies	Tryptophan Degradation To 2-amino-3-carboxymuconate Semialdehyde Pathway Diagram

Diseases associated with anti-Kynu antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>3 publications with Kynu and Cardiovascular Diseases
Nervous System Diseases Antibodies	>2 publications with Kynu and Nervous System Diseases
Congenital Abnormalities Antibodies	>1 publications with Kynu and Congenital Abnormalities
Neoplasms Antibodies	>1 publications with Kynu and Neoplasms
Necrosis Antibodies	>1 publications with Kynu and Necrosis
Hydroxykynureninuria Antibodies	>1 publications with Kynu and Hydroxykynureninuria

Organs/Tissues associated with anti-Kynu antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1 publications with Kynu and Liver