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ATG5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641681

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves… More details

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Product Overview

Online Datasheet in PDF
Product Name
APG5 (ATG5), Polyclonal Antibody
Full Product Name
APG5 (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein)
Product Gene Name
anti-ATG5 antibody
Product Synonym Names
Anti -APG5 (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
32,447 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (106632351..106773695, complement). Location: 6q21
OMIM
604261
3D Structure
ModBase 3D Structure for Q9H1Y0
Immunogen
APG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human APG5.

Product Categories/Family for anti-ATG5 antibody

Antibodies; Abs to Autophagy
Applications Tested/Suitable for anti-ATG5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ATG5 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA1:1,000
Western blotting: 1:100-500
Immunohistochemistry: 1:10-50

Immunofluorescence (IF) of anti-ATG5 antibody

Fluorescent image of U251 cells stained with APG5 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812c APG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Rabbit Anti-ATG5 Polyclonal Antibody – Cat# MBS641681

Western Blot (WB) of anti-ATG5 antibody

APG5 Antibody western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the APG5 antibody detected the APG5 protein (arrow).
Rabbit Anti-ATG5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS641681

Immunohistochemistry (IHC) of anti-ATG5 antibody

APG5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG5 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-ATG5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS641681

Other Notes

Small volumes of anti-ATG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG5 purchase

MBS641681 is an antibody targeting ATG5. Also known as APG5 (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG5. It may not necessarily be applicable to this product.

NCBI GI #
4757798
NCBI GeneID
9474
NCBI Accession #
NP_004840.1
UniProt Primary Accession #
Q9H1Y0
UniProt Secondary Accession #
O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
UniProt Related Accession #
Q9H1Y0
GenBank Nucleotide #
NM_004849.2
NCBI Official Full Name
autophagy protein 5
NCBI Official Synonym Full Names
autophagy related 5
NCBI Official Symbol
ATG5
NCBI Official Synonym Symbols
ASP; APG5; APG5L; hAPG5; APG5-LIKE
UniProt Protein Name
Autophagy protein 5
UniProt Synonym Protein Names
APG5-like; Apoptosis-specific protein
Protein Family
Autophagy protein
UniProt Gene Name
ATG5
UniProt Synonym Gene Names
APG5L; ASP
UniProt Entry Name
ATG5_HUMAN
NCBI Protein Information
autophagy protein 5; apoptosis specific protein; apoptosis-specific protein; ATG5 autophagy related 5 homolog

UniProt Comments for ATG5

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; innate immune response; autophagy; blood vessel remodeling; negative regulation of protein ubiquitination; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation
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Research Articles on ATG5

1. MIR181A is a novel and important regulator of autophagy and ATG5 is a rate-limiting miRNA target in this effect

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG5 antibody

 Reference Product  PubMed Publications
 ATG12 antibody  >156 publications with ATG5 and ATG12
 ATG3 antibody  >36 publications with ATG5 and ATG3
 ATG10 antibody  >31 publications with ATG5 and ATG10
 ATG16L1 antibody  >27 publications with ATG5 and ATG16L1
 CASP8 antibody  >20 publications with ATG5 and CASP8
 FADD antibody  >12 publications with ATG5 and FADD
 DDX58 antibody  >5 publications with ATG5 and DDX58
 SIRT1 antibody  >4 publications with ATG5 and SIRT1
 MAVS antibody  >3 publications with ATG5 and MAVS
 WDFY3 antibody  >1 publications with ATG5 and WDFY3

Pathways associated with anti-ATG5 antibody

 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways antibodies  RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Shigellosis Pathway antibodies  Shigellosis Pathway Diagram

Diseases associated with anti-ATG5 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >182 publications with ATG5 and Neoplasms
 Necrosis Antibodies  >43 publications with ATG5 and Necrosis
 Cardiovascular Diseases Antibodies  >38 publications with ATG5 and Cardiovascular Diseases
 Inflammation Antibodies  >32 publications with ATG5 and Inflammation
 Gastrointestinal Diseases Antibodies  >22 publications with ATG5 and Gastrointestinal Diseases
 Melanoma Antibodies  >21 publications with ATG5 and Melanoma
 Carcinoma, Hepatocellular Antibodies  >19 publications with ATG5 and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >17 publications with ATG5 and Breast Neoplasms
 Liver Neoplasms Antibodies  >16 publications with ATG5 and Liver Neoplasms
 Prostatic Neoplasms Antibodies  >13 publications with ATG5 and Prostatic Neoplasms

Organs/Tissues associated with anti-ATG5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >62 publications with ATG5 and Brain
 Blood Antibodies  >51 publications with ATG5 and Blood
 Liver Antibodies  >43 publications with ATG5 and Liver
 Muscle Antibodies  >39 publications with ATG5 and Muscle
 Lung Antibodies  >37 publications with ATG5 and Lung
 Heart Antibodies  >34 publications with ATG5 and Heart
 Kidney Antibodies  >22 publications with ATG5 and Kidney
 Eye Antibodies  >21 publications with ATG5 and Eye
 Embryonic Tissue Antibodies  >21 publications with ATG5 and Embryonic Tissue
 Prostate Antibodies  >19 publications with ATG5 and Prostate
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