Mouse Anti-B3GNT1 Monoclonal Antibody – Cat# MBS641716

Detection of Human B-1,3-N-acetylglucosaminyltransferase 1/B3GNT1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 2ug/ml of Mouse Human B-1,3-N- acetylglucosaminyl-transferase 1/B3GNT1 Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for B-1,3-N- acetylglucosaminyltransferase 1/B3GNT1 at approximately 55kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

B3GNT1 Antibody – Mouse Monoclonal

Name: B3GNT1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS641716
Price: 955 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641716

B3GNT1 is a 50kD member of the b3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be… More details

$955 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

B3GNT1, Monoclonal Antibody

Full Product Name

B3GNT1 (N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, iGnT, Poly-N-acetyllactosamine Extension Enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, B3GNT6)

Product Gene Name

anti-B3gnt1 antibody

Product Synonym Names

Anti -B3GNT1 (N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, iGnT, Poly-N-acetyllactosamine Extension Enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, B3GNT6)

Clonality

Monoclonal

Isotype

IgG2B

Clone Number

12K14

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

Purity/Purification

Affinity Purified
Purified by Protein A/G affinity chromatography.

Specificity

Recognizes human B3GNT1.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

47,384 Da

Chromosome Location

Chromosome: 19; NC_000085.6 (5038826..5041134). Location: 19 A; 19

3D Structure

ModBase 3D Structure for Q8BWP8

Immunogen

Recombinant corresponding to aa43-415 from human B3GNT1 expressed in CHO cell line.

Product Categories/Family for anti-B3gnt1 antibody

Antibodies; Abs to Enzymes; Glycosyltransferase

Applications Tested/Suitable for anti-B3gnt1 antibody

Western Blot (WB)

Application Notes for anti-B3gnt1 antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 2ug/ml

Other Notes

Small volumes of anti-B3gnt1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for B3gnt1 purchase

MBS641716 is an antibody targeting B3gnt1. Also known as B3GNT1 (N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, iGnT, Poly-N-acetyllactosamine Extension Enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, B3GNT6). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for B3gnt1. It may not necessarily be applicable to this product.

NCBI GI #

47124409

NCBI GeneID

108902

NCBI Accession #

AAH69927.1

UniProt Primary Accession #

Q8BWP8

UniProt Secondary Accession #

Q3TY43; Q8BJH9; Q99LW7

UniProt Related Accession #

Q8BWP8

NCBI Official Full Name

B3gnt1 protein

NCBI Official Synonym Full Names

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1

NCBI Official Symbol

B3gnt1

NCBI Official Synonym Symbols

iGAT; iGNT; B3gnt6; BETA3GNT1; 1500032M01Rik

UniProt Protein Name

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase

UniProt Synonym Protein Names

I-beta-1,3-N-acetylglucosaminyltransferase; iGnT; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1

UniProt Gene Name

B3gnt1

UniProt Synonym Gene Names

B3gnt6; iGnT

UniProt Entry Name

B3GN1_MOUSE

NCBI Protein Information

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; poly-N-acetyllactosamine extension enzyme; I-beta-1,3-N-acetylglucosaminyltransferase; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6

UniProt Comments for B3gnt1

B4GAT1: Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase; Membrane protein, integral; Glycan Metabolism - keratan sulfate biosynthesis; EC 2.4.1.149

Cellular Component: Golgi apparatus; membrane; integral to membrane

Molecular Function: glucuronosyltransferase activity; oligosaccharyl transferase activity; alpha(1,6)-fucosyltransferase activity; metal ion binding; acetylgalactosaminyltransferase activity; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity; alpha(1,3)-fucosyltransferase activity; glucosyltransferase activity; O antigen polymerase activity; alpha-1,2-mannosyltransferase activity; acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups; 1-phenanthrol glycosyltransferase activity; phenanthrol glycosyltransferase activity; glycolipid mannosyltransferase activity; galactosyltransferase activity; dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity; fucosyltransferase activity; dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity; lipopolysaccharide-1,6-galactosyltransferase activity; alpha-1,2-galactosyltransferase activity; alpha-1,3-galactosyltransferase activity; dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; 9-phenanthrol UDP-glucuronosyltransferase activity; UDP-glucose:glycoprotein glucosyltransferase activity; 1,2-dihydroxy-phenanthrene glycosyltransferase activity; 9-phenanthrol glycosyltransferase activity; transferase activity; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; cellulose synthase activity; beta-1,4-mannosyltransferase activity; mannosyltransferase activity; alpha-1,3-mannosyltransferase activity; alpha-1,6-mannosyltransferase activity; inositol phosphoceramide synthase activity

Biological Process: axon guidance; protein amino acid O-linked mannosylation; protein amino acid glycosylation

Research Articles on B3gnt1

1. This study demonistrated that the axonal guidance defects observed in B3gnt1 mutant mice

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-B3gnt1 antibody

Reference Product	PubMed Publications
Fut9 antibody	>1 publications with B3gnt1 and Fut9

Pathways associated with anti-B3gnt1 antibody

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies	Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies	Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
Glycosaminoglycan Metabolism Pathway antibodies	Glycosaminoglycan Metabolism Pathway Diagram
Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies	Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies	Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
Keratan Sulfate Biosynthesis Pathway antibodies	Keratan Sulfate Biosynthesis Pathway Diagram
Keratan Sulfate/keratin Metabolism Pathway antibodies	Keratan Sulfate/keratin Metabolism Pathway Diagram
MPS I - Hurler Syndrome Pathway antibodies	MPS I - Hurler Syndrome Pathway Diagram
MPS II - Hunter Syndrome Pathway antibodies	MPS II - Hunter Syndrome Pathway Diagram

Diseases associated with anti-B3gnt1 antibody

Disease Name	Pubmed Publications
Congenital Abnormalities Antibodies	>1 publications with B3gnt1 and Congenital Abnormalities

Organs/Tissues associated with anti-B3gnt1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with B3gnt1 and Brain
Eye Antibodies	>1 publications with B3gnt1 and Eye
Testis Antibodies	>1 publications with B3gnt1 and Testis
Kidney Antibodies	>1 publications with B3gnt1 and Kidney
Embryonic Tissue Antibodies	>1 publications with B3gnt1 and Embryonic Tissue