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IL36A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641844

MBS641844 is an antibody targeting IL36A. Also known as IL1F6, ID (IL36A, FIL1E, IL1E, IL1F6, Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, Interleukin-1 family member 6). Reactive in Human. Host: Rabbit. The function of this protein has not been… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IL1F6 (IL36A), Polyclonal Antibody
Full Product Name
IL1F6, ID (IL36A, FIL1E, IL1E, IL1F6, Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, Interleukin-1 family member 6)
Product Gene Name
anti-IL36A antibody
Product Synonym Names
Anti -IL1F6, ID (IL36A, FIL1E, IL1E, IL1F6, Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, Interleukin-1 family member 6)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
17,684 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (113763038..113766221). Location: 2q12-q14.1
OMIM
605509
3D Structure
ModBase 3D Structure for Q9UHA7
Immunogen
IL1F6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-73 amino acids from the Central region of human IL1F6.

Product Categories/Family for anti-IL36A antibody

Antibodies; Abs to Interleukin
Applications Tested/Suitable for anti-IL36A antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-IL36A antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-IL36A antibody

Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) using MBS641844. IL1F6 (arrow) was detected using the purified Pab.
Rabbit Anti-IL36A Polyclonal Antibody – Cat# MBS641844

Immunohistochemistry (IHC) of anti-IL36A antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue using MBS641844 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS641844 for immunohistochemistry.
Rabbit Anti-IL36A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS641844

Flow Cytometry (FC/FACS) of anti-IL36A antibody

Flow Cytometric analysis of MDA-MB231 cells using MBS641844 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-IL36A Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS641844

Other Notes

Small volumes of anti-IL36A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL36A. It may not necessarily be applicable to this product.

NCBI GI #
7657092
NCBI GeneID
27179
NCBI Accession #
NP_055255.1
UniProt Primary Accession #
Q9UHA7
UniProt Secondary Accession #
Q53SR7; Q5BLR4; Q7RTZ8; B2RAD9
UniProt Related Accession #
Q9UHA7
GenBank Nucleotide #
NM_014440.1
NCBI Official Full Name
interleukin-36 alpha
NCBI Official Synonym Full Names
interleukin 36, alpha
NCBI Official Symbol
IL36A
NCBI Official Synonym Symbols
FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
UniProt Protein Name
Interleukin-36 alpha
UniProt Synonym Protein Names
FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6
Protein Family
Interleukin
UniProt Gene Name
IL36A
UniProt Synonym Gene Names
FIL1E; IL1E; IL1F6; IL-1 epsilon; IL-1F6
UniProt Entry Name
IL36A_HUMAN
NCBI Protein Information
interleukin-36 alpha; FIL1 epsilon; IL-1 epsilon; interleukin-1 epsilon; IL-1F6 (FIL-1-epsilon); interleukin 1, epsilon; interleukin-1 family member 6; interleukin 1 family, member 6 (epsilon)

UniProt Comments for IL36A

IL1F6: Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q12-q14.1

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-1 receptor binding; cytokine activity

Biological Process: cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of interleukin-6 production; immune response; inflammatory response
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Research Articles on IL36A

1. Interleukin-36 (IL-36) ligands require processing for full agonist (IL-36alpha, IL-36beta, and IL-36gamma) or antagonist (IL-36Ra) activity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-IL36A antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >1 publications with IL36A and Immune System Diseases
 Necrosis Antibodies  >1 publications with IL36A and Necrosis
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