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CD247 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS641854

T-cell receptor zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD3Z (CD247), Polyclonal Antibody
Full Product Name
CD3Z, NT (CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247)
Product Gene Name
anti-CD247 antibody
Product Synonym Names
Anti -CD3Z, NT (CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
18,696 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (167399877..167487847, complement). Location: 1q24.2
OMIM
186780
3D Structure
ModBase 3D Structure for P20963
Immunogen
CD3Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10~39 amino acids from the N-terminal region of human CD3Z.

Product Categories/Family for anti-CD247 antibody

Antibodies; Abs to CD Markers
Applications Tested/Suitable for anti-CD247 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-CD247 antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100

Western Blot (WB) of anti-CD247 antibody

Western blot analysis of anti-CD3Z Antibody (N-term) (RB13817) in Ramos cell line lysates (35ug/lane). CD3Z(arrow) was detected using the purified Pab.
Rabbit Anti-CD247 Polyclonal Antibody – Cat# MBS641854

Flow Cytometry (FC/FACS) of anti-CD247 antibody

Flow cytometric analysis of Ramos cells using CD3Z Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CD247 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS641854

Other Notes

Small volumes of anti-CD247 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD247 purchase

MBS641854 is an antibody targeting CD247. Also known as CD3Z, NT (CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD247. It may not necessarily be applicable to this product.

NCBI GI #
66841741
NCBI GeneID
919
NCBI Accession #
AAY57330.1
UniProt Primary Accession #
P20963
UniProt Secondary Accession #
Q5VX13; Q8TAX4; B1AK49
UniProt Related Accession #
P20963; A46461
NCBI Official Full Name
CD3Z antigen, zeta polypeptide (TiT3 complex)
NCBI Official Synonym Full Names
CD247 molecule
NCBI Official Symbol
CD247
NCBI Official Synonym Symbols
T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
UniProt Protein Name
T-cell surface glycoprotein CD3 zeta chain
UniProt Synonym Protein Names
T-cell receptor T3 zeta chain
Protein Family
T-cell surface glycoprotein
UniProt Gene Name
CD247
UniProt Synonym Gene Names
CD3Z; T3Z; TCRZ
UniProt Entry Name
CD3Z_HUMAN
NCBI Protein Information
T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3

NCBI Summary for CD247

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD247

CD3Z: a T cell surface glycoprotein that is a component of the T cell antigen receptor. Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD3-epsilon results in impaired immune response. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; protein homodimerization activity

Biological Process: regulation of immune response; viral reproduction; T cell costimulation; innate immune response; T cell receptor signaling pathway; regulation of defense response to virus by virus

Disease: Immunodeficiency 25
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Research Articles on CD247

1. Low expression of T-cell antigen receptor complex zeta is associated with chronic myeloid leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD247 antibody

 Reference Product  PubMed Publications
 ZAP70 antibody  >2 publications with CD247 and ZAP70
 FYN antibody  >1 publications with CD247 and FYN
 SHC1 antibody  >1 publications with CD247 and SHC1
 CD3G antibody  >1 publications with CD247 and CD3G
 CD3D antibody  >1 publications with CD247 and CD3D
 LCK antibody  >1 publications with CD247 and LCK

Pathways associated with anti-CD247 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 FCGR Activation Pathway antibodies  FCGR Activation Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram

Diseases associated with anti-CD247 antibody

 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >7 publications with CD247 and Autoimmune Diseases
 Immune System Diseases Antibodies  >6 publications with CD247 and Immune System Diseases
 Neoplasms Antibodies  >4 publications with CD247 and Neoplasms
 Scleroderma, Systemic Antibodies  >4 publications with CD247 and Scleroderma, Systemic
 Inflammation Antibodies  >3 publications with CD247 and Inflammation
 Carcinoma Antibodies  >1 publications with CD247 and Carcinoma
 Carcinoma, Squamous Cell Antibodies  >1 publications with CD247 and Carcinoma, Squamous Cell
 Cardiovascular Diseases Antibodies  >1 publications with CD247 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >1 publications with CD247 and Breast Neoplasms
 Hypertension Antibodies  >1 publications with CD247 and Hypertension

Organs/Tissues associated with anti-CD247 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11 publications with CD247 and Blood
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