PATJ Antibody – Goat Polyclonal

Function: Involved in cell polarity establishment. Probably participates in the assembly, positioning and maintenance of adherens junctions via its interaction with the SAC complex. Ref.9

Subunit structure: Component of the SAC complex, a complex composed of crb, Patj and sdt. Interacts directly with nrx via its third and fourth PDZ domains. Interacts directly with par-6, possibly mediating a link between the SAC complex and the par-6 complex, which is composed of par-6, baz and aPKC. Ref.1 Ref.3 Ref.7 Ref.8

Subcellular location: Membrane. Note: Membrane-associated. Localizes to the subapical domain of cells. Sdt is required for its localization. At mitotic cycles 13 and 14, during cellularization, it localizes at the leading edge of the invaginating membranes. It is then exclusively localized to the basal area of columnal epithelial cells. In the germline, it localizes at the membrane of the nurse cells during early and mid oogenesis. Later, it is found in the nuclear membrane in close association with actin filaments that connect the nuclei of the nurse cells with the plasma membrane. Ref.1 Ref.3

Tissue specificity: Expressed in primary and some secondary epithelial cells such as ectodermal cells, salivary glands, foregut, hindgut and invaginating tracheal cells. Also expressed in specific cells of the peripheral nervous system. Expressed in the germline. Ref.1 Ref.3

Developmental stage: Expressed both maternally and zygotically. Appears in mitotic cycle 11 when the furrows made by the imprint of the embryonic nuclei become apparent. Ref.1

Sequence similarities: Belongs to the Patj family.Contains 1 L27 domain.Contains 4 PDZ (DHR) domains.

Caution: Was originally thought to be the Disks lost (Dlt) protein. However, Ref.9 showed that it is not the case and renamed it Patj. This drastically changes the first conclusions drawn about its essential function, since the mutant used contained defects for another protein, which is now called Dlt. If its association with proteins involved in cell polarization complexes is clear, Patj is not essential since its absence apparently does not lead to important defects.

Sequence caution: The sequence AAD43031.1 differs from that shown. Reason: Erroneous initiation.