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HMGB3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642054

HMGB3, also known as High mobility group protein B3, is a DNA associated protein that binds preferentially single-stranded DNA and unwinds double stranded DNA. This transcription factor belongs to the HMGB family and high mobility group (HMG) protein… More details

$710 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HMG4 (HMGB3), Polyclonal Antibody
Full Product Name
HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)
Product Gene Name
Product Synonym Names
Anti -HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine, Canine, Chicken, Equine, Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human HMGB3. Species Crossreactivity: chicken, chimpanzee, bovine, canine, equine, mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
22,980 Da
Chromosome Location
Chromosome: X; NC_000023.10 (150149056..150159248). Location: Xq28
OMIM
300193
3D Structure
ModBase 3D Structure for O15347
Immunogen
Synthetic peptide corresponding to aa150-200 of human HMGB3.
Positive Control
Mouse placenta
Applications Tested/Suitable for anti-HMGB3 antibody
Western Blot (WB)
Application Notes for anti-HMGB3 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 0.5-1ug/ml

Western Blot (WB) of anti-HMGB3 antibody

Western blot analysis of HMGB3 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using MBS642054 at 0.5ug/ml. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.
Rabbit Anti-HMGB3 Polyclonal Antibody – Cat# MBS642054

Other Notes

Small volumes of anti-HMGB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HMGB3 purchase

MBS642054 is an antibody targeting HMGB3. Also known as HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4). Reactive in Bovine, Canine, Chicken, Equine, Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HMGB3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
high mobility group protein B3
NCBI Official Synonym Full Names
high mobility group box 3
NCBI Official Symbol
HMGB3
NCBI Official Synonym Symbols
HMG4; HMG-4; HMG2A; HMG-2a
UniProt Protein Name
High mobility group protein B3
UniProt Synonym Protein Names
High mobility group protein 2a; HMG-2a; High mobility group protein 4
UniProt Gene Name
HMGB3
UniProt Synonym Gene Names
HMG2A; HMG4; HMG-2a; HMG-4
UniProt Entry Name
HMGB3_HUMAN
NCBI Protein Information
high mobility group protein B3; high-mobility group box 3; high mobility group protein 4; high mobility group protein 2a; non-histone chromosomal protein; high-mobility group (nonhistone chromosomal) protein 4

NCBI Summary for HMGB3

HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM, Mar 2008]
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UniProt Comments for HMGB3

HMGB3: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Belongs to the HMGB family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: chromosome; nucleus

Molecular Function: double-stranded DNA binding; chromatin binding; DNA bending activity

Biological Process: negative regulation of B cell differentiation; chromatin remodeling; negative regulation of myeloid cell differentiation; regulation of transcription, DNA-dependent; multicellular organismal development; DNA recombination

Disease: Microphthalmia, Syndromic 13
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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