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Tudor Domain and Kh Domain Containing Protein, TDRD2 (TDRKH) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642126

TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70kD member of the Tudor family of proteins. It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells. TDRKH forms part of an… More details

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Product Overview

Online Datasheet in PDF
Product Name
TDRKH, Tudor domain and KH domain containing protein, TDRD2, Polyclonal Antibody
Full Product Name
TDRKH, Tudor domain and KH domain containing protein, TDRD2
Product Gene Name
anti-TDRKH antibody
Product Synonym Names
Anti -TDRKH, Tudor domain and KH domain containing protein, TDRD2
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with sterile PBS.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TDRKH.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
62,046 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (151742741..151763052, complement). Location: 1q21
OMIM
609501
3D Structure
ModBase 3D Structure for Q9Y2W6
Immunogen
Recombinant corresponding to His206-Leu561 form human TDRKH expressed in E. coli (Q9Y2W6).

Product Categories/Family for anti-TDRKH antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-TDRKH antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TDRKH antibody
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry: 5-15ug/ml
Western Blot: 1ug/ml

Western Blot (WB) of anti-TDRKH antibody

Detection of Human TDRKH by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1ug/ml of Human TDRKH Antigen Affinity­purified Polyclonal Antibody followed by HRP­conjugated Sheep IgG Secondary Antibody A specific band was detected for TDRKH at approximately 70kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Sheep Anti-TDRKH Polyclonal Antibody – Cat# MBS642126

Immunohistochemistry (IHC) of anti-TDRKH antibody

TDRKH in Mouse Testis. TDRKH was detected in immersion fixed frozen sections of adult mouse testis using Human TDRKH Affinity­purified Polyclonal Antibody at 10ug/ml overnight at 4 °C. Tissue was stained using the NorthernLightsTM 493­conjugated Sheep IgG Secondary Antibody and counterstained with DAPI (blue). Specific staining was localized to germ cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Sheep Anti-TDRKH Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS642126

Other Notes

Small volumes of anti-TDRKH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TDRKH purchase

MBS642126 is an antibody targeting TDRKH. Also known as TDRKH, Tudor domain and KH domain containing protein, TDRD2. Reactive in Human. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for TDRKH. It may not necessarily be applicable to this product.

NCBI GI #
145312270
NCBI GeneID
11022
NCBI Accession #
NP_001077432.1
UniProt Primary Accession #
Q9Y2W6
UniProt Secondary Accession #
Q5SZR3; Q5SZR5; Q8N582; Q9NYV5; D3DV24
UniProt Related Accession #
Q9Y2W6
GenBank Nucleotide #
NM_001083963.1
NCBI Official Full Name
tudor and KH domain-containing protein isoform a
NCBI Official Synonym Full Names
tudor and KH domain containing
NCBI Official Symbol
TDRKH
NCBI Official Synonym Symbols
TDRD2
UniProt Protein Name
Tudor and KH domain-containing protein
UniProt Synonym Protein Names
Tudor domain-containing protein 2
Protein Family
Tudor and KH domain-containing protein
UniProt Gene Name
TDRKH
UniProt Synonym Gene Names
TDRD2
UniProt Entry Name
TDRKH_HUMAN
NCBI Protein Information
tudor and KH domain-containing protein; tudor domain containing 2; putative RNA binding protein; tudor domain-containing protein 2; tudor and KH domain containing protein

UniProt Comments for TDRKH

TDRKH: a protein containing one Tudor and two KH_1 domims. Tudor domains have been shown to interact with arginine-glycine-rich motifs in a methylarginine-dependent manner, and target methylated arginines physiologically. KH domains are RNA-binding domains found in a wide variety of nucleic acid-binding proteins. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: mitochondrion

Molecular Function: RNA binding

Biological Process: fertilization; DNA methylation during gametogenesis; spermatogenesis; gene expression; RNA-mediated gene silencing; cell differentiation; male meiosis
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Research Articles on TDRKH

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TDRKH antibody

 Reference Product  PubMed Publications
 PIWIL1 antibody  >2 publications with TDRKH and PIWIL1
 PIWIL4 antibody  >1 publications with TDRKH and PIWIL4
 CLNS1A antibody  >1 publications with TDRKH and CLNS1A

Organs/Tissues associated with anti-TDRKH antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with TDRKH and Lung
 Embryonic Tissue Antibodies  >1 publications with TDRKH and Embryonic Tissue
 Testis Antibodies  >1 publications with TDRKH and Testis
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