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TRMT112 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642316

Participates both in methylation of protein and tRNA species. The heterodimer with HEMK2/N6AMT1 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. The heterodimer with ALKBH8 catalyzes the methylation of… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TRMT112, Monoclonal Antibody
Full Product Name
TRMT112 (tRNA Methyltransferase 112 Homolog, TRM112-like Protein, AD-001, HSPC152, HSPC170)
Product Gene Name
Product Synonym Names
Anti -TRMT112 (tRNA Methyltransferase 112 Homolog, TRM112-like Protein, AD-001, HSPC152, HSPC170)
Immunogen Sequence
MKLLTHNLLS SHVRGVGSRG FPLRLQATEV RICPVEFNPN FVARMIPKVE WSAFLEAADN LRLIQVPKGP VEGYEENEEF LRTMHHLLLE VEVIEGTLQC PESGRMFPIS RGIPNMLLSE EETES
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3E5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human HSPC152.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
14,141 Da
Chromosome Location
Chromosome: 19; NC_000085.6 (6909698..6911028). Location: 19; 19 E1
3D Structure
ModBase 3D Structure for Q9DCG9
Immunogen
Full length recombinant corresponding to aa1-126 from human HSPC152 (AAH17172) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-Trmt112 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Trmt112 antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-Trmt112 antibody

Western Blot detection against Immunogen (39.86kD).
Mouse Anti-TRMT112 Monoclonal Antibody – Cat# MBS642316

Western Blot (WB) of anti-Trmt112 antibody

Western Blot analysis of HSPC152 expression in transfected 293T cell line by HSPC152 monoclonal antibody.|Lane 1: HSPC152 transfected lysate (14.2kD).|Lane 2: Non-transfected lysate.
Mouse Anti-TRMT112 Monoclonal Antibody, Western Blot (WB) – Cat# MBS642316

Other Notes

Small volumes of anti-Trmt112 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Trmt112 purchase

MBS642316 is an antibody targeting Trmt112. Also known as TRMT112 (tRNA Methyltransferase 112 Homolog, TRM112-like Protein, AD-001, HSPC152, HSPC170). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Trmt112. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tRNA methyltransferase 112 homolog
NCBI Official Synonym Full Names
tRNA methyltransferase 11-2
NCBI Official Symbol
Trmt112
NCBI Official Synonym Symbols
Trm112p; 0610038D11Rik
UniProt Protein Name
tRNA methyltransferase 112 homolog
UniProt Synonym Protein Names
TRM112-like protein
UniProt Gene Name
Trmt112
UniProt Entry Name
TR112_MOUSE
NCBI Protein Information
tRNA methyltransferase 112 homolog; TRM112-like protein; tRNA methyltransferase 11-2 homolog

UniProt Comments for Trmt112

HSPC152: Participates both in methylation of protein and tRNA species. The heterodimer with HEMK2/N6AMT1 catalyzes N5- methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species. Belongs to the TRM112 family.

Cellular Component: protein complex

Molecular Function: protein methyltransferase activity

Biological Process: peptidyl-glutamine methylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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