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HMGB3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642473

High mobility group protein B3 is a 23kD nuclear protein and member of the HMGB family. Human HMGB3 is synthesized as a 200aa precursor, which is demethylated to produce the 199aa mature chain. The protein contains two HMG box DNA-binding domains… More details

$970 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HMG4 (HMGB3), Monoclonal Antibody
Full Product Name
HMG4 (High Mobility Group 4 (HMG) Transcription Factor, HMG 4, HMG-4, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)
Product Gene Name
Product Synonym Names
Anti -HMG4 (High Mobility Group 4 (HMG) Transcription Factor, HMG 4, HMG-4, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
11n84
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Purity/Purification
Affinity Purified
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human HMGB3. Species Crossreactivity: mouse. No cross-reactivity with recombinant human HMG-1, A1A, A1B, or A2.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
22,980 Da
Chromosome Location
Chromosome: X; NC_000023.10 (150149056..150159248). Location: Xq28
OMIM
300193
3D Structure
ModBase 3D Structure for O15347
Immunogen
Recombinant corresponding to aa1-180 from human HMGB3 expressed in E. coli (O15347).
Applications Tested/Suitable for anti-HMGB3 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-HMGB3 antibody
Suitable for use in Western Blot and Immunocytochemistry.
Dilution: Western Blot: 2ug/ml
Immunocytochemistry: 8-25ug/ml

Western Blot (WB) of anti-HMGB3 antibody

Detection of Human HMGB3 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 2ug/ml of Human/Mouse HMGB3 Monoclonal Antibody followed by HRP­conjugated Mouse IgG Secondary Antibody. A specific band was detected for HMGB3 at approximately 23kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Mouse Anti-HMGB3 Monoclonal Antibody – Cat# MBS642473

Testing Data of anti-HMGB3 antibody

HMGB3 in Detroit 551 Human Cell Line. HMGB3 was detected in immersion fixed Detroit 551 human skin fibroblast cell line using Human/Mouse HMGB3 Monoclonal Antibody at 10ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLightsTM 557­conjugated Mouse IgG Secondary Antibody and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Mouse Anti-HMGB3 Monoclonal Antibody, Testing Data – Cat# MBS642473

Other Notes

Small volumes of anti-HMGB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HMGB3 purchase

MBS642473 is an antibody targeting HMGB3. Also known as HMG4 (High Mobility Group 4 (HMG) Transcription Factor, HMG 4, HMG-4, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HMGB3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
high mobility group protein B3
NCBI Official Synonym Full Names
high mobility group box 3
NCBI Official Symbol
HMGB3
NCBI Official Synonym Symbols
HMG4; HMG-4; HMG2A; HMG-2a
UniProt Protein Name
High mobility group protein B3
UniProt Synonym Protein Names
High mobility group protein 2a; HMG-2a; High mobility group protein 4
UniProt Gene Name
HMGB3
UniProt Synonym Gene Names
HMG2A; HMG4; HMG-2a; HMG-4
UniProt Entry Name
HMGB3_HUMAN
NCBI Protein Information
high mobility group protein B3; high-mobility group box 3; high mobility group protein 4; high mobility group protein 2a; non-histone chromosomal protein; high-mobility group (nonhistone chromosomal) protein 4

NCBI Summary for HMGB3

HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM, Mar 2008]
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UniProt Comments for HMGB3

HMGB3: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Belongs to the HMGB family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: chromosome; nucleus

Molecular Function: double-stranded DNA binding; chromatin binding; DNA bending activity

Biological Process: chromatin remodeling; negative regulation of B cell differentiation; negative regulation of myeloid cell differentiation; regulation of transcription, DNA-dependent; multicellular organismal development; DNA recombination

Disease: Microphthalmia, Syndromic 13
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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