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GUCY2D Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642483

This gene encodes a retina-specific guanylate cyclase, which is a member of the membrane guanylyl cyclase family. Like other membrane guanylyl cyclases, this enzyme has a hydrophobic amino-terminal signal sequence followed by a large extracellular domain, a… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GUCY2D, Polyclonal Antibody
Full Product Name
GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase)
Product Synonym Names
Anti -GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
GUCY2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547-578 amino acids from the Central region of human GUCY2D.

Product Categories/Family for anti-GUCY2D antibody

Applications Tested/Suitable for anti-GUCY2D antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-GUCY2D antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-GUCY2D antibody

Western Blot analysis in Ramos cell line lysates (35ug/lane) using MBS642483. This demonstrates that MBS642483 detected the GUCY2D protein (arrow).
Rabbit Anti-GUCY2D Polyclonal Antibody – Cat# MBS642483

Western Blot (WB) of anti-GUCY2D antibody

Western Blot analysis in rat eyeball lysate (20ug/lane) using MBS642483 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 120kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-GUCY2D Polyclonal Antibody, Western Blot (WB) – Cat# MBS642483

Immunohistochemistry (IHC) of anti-GUCY2D antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue using MBS642483 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642483 for immunohistochemistry.
Rabbit Anti-GUCY2D Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS642483

Testing Data of anti-GUCY2D antibody

Overlay histogram showing Hela cells stained with MBS642483 (green line). The cells were fixed with 2% paraformaldehyde (10mins). The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by MBS642483 (1:25) for 60mins at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40mins at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Anti-GUCY2D Polyclonal Antibody, Testing Data – Cat# MBS642483

Other Notes

Small volumes of anti-GUCY2D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GUCY2D purchase

MBS642483 is an antibody targeting GUCY2D. Also known as GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GUCY2D. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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