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POFUT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642582

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POFUT2, Polyclonal Antibody
Full Product Name
POFUT2, ID (POFUT2, C21orf80, FUT13, KIAA0958, GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2)
Product Gene Name
Product Synonym Names
Anti -POFUT2, ID (POFUT2, C21orf80, FUT13, KIAA0958, GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
49,976 Da
Chromosome Location
Chromosome: 21; NC_000021.8 (46683843..46707811, complement). Location: 21q22.3
OMIM
610249
3D Structure
ModBase 3D Structure for Q9Y2G5
Immunogen
POFUT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-265 amino acids from the Central region of human POFUT2.
Applications Tested/Suitable for anti-POFUT2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-POFUT2 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-POFUT2 antibody

POFUT2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the POFUT2 antibody detected the POFUT2 protein (arrow).
Rabbit Anti-POFUT2 Polyclonal Antibody – Cat# MBS642582

Immunohistochemistry (IHC) of anti-POFUT2 antibody

POFUT2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POFUT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-POFUT2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS642582

Other Notes

Small volumes of anti-POFUT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POFUT2 purchase

MBS642582 is an antibody targeting POFUT2. Also known as POFUT2, ID (POFUT2, C21orf80, FUT13, KIAA0958, GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POFUT2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
POFUT2 protein
NCBI Official Synonym Full Names
protein O-fucosyltransferase 2
NCBI Official Symbol
POFUT2
NCBI Official Synonym Symbols
FUT13; C21orf80
UniProt Protein Name
GDP-fucose protein O-fucosyltransferase 2
UniProt Synonym Protein Names
Peptide-O-fucosyltransferase 2
UniProt Gene Name
POFUT2
UniProt Synonym Gene Names
C21orf80; FUT13; KIAA0958; O-FucT-2
UniProt Entry Name
OFUT2_HUMAN
NCBI Protein Information
GDP-fucose protein O-fucosyltransferase 2; O-FucT-2; peptide-O-fucosyltransferase 2

UniProt Comments for POFUT2

POFUT2: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Belongs to the glycosyltransferase 68 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.4.1.221

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane

Molecular Function: peptide-O-fucosyltransferase activity

Biological Process: fucose metabolic process; protein amino acid O-linked glycosylation; cellular protein metabolic process; mesoderm formation; regulation of gene expression; regulation of secretion; post-translational protein modification
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POFUT2 antibody

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