Mouse Anti-THG1L Monoclonal Antibody – Cat# MBS642601

Western Blot analysis of THG1L expression in transfected 293T cell line by THG1L monoclonal antibody.|Lane 1: THG1L transfected lysate (34.8kD).|Lane 2: Non-transfected lysate.

THG1L Antibody – Mouse Monoclonal

Name: THG1L Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS642601
Price: 880 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642601

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. More details

$880 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

THG1L, Monoclonal Antibody

Full Product Name

THG1L (Probable tRNA(His) Guanylyltransferase, tRNA-histidine Guanylyltransferase, IHG-1, Interphase Cytoplasmic Foci Protein 45, ICF45)

Product Gene Name

anti-THG1L antibody

Product Synonym Names

Anti -THG1L (Probable tRNA(His) Guanylyltransferase, tRNA-histidine Guanylyltransferase, IHG-1, Interphase Cytoplasmic Foci Protein 45, ICF45)

Immunogen Sequence

MAKSKFEYVR DFEADDTCLA HCWVVVRLDG RNFHRFAEKH NFAKPNDSRA LQLMTKCAQT VMEELEDIVI AYGQSDEYSF VFKRKTNWFK RRASKFMTHV ASQFASSYVF YWRDYFEDQP LLYPPGFDGR VVVYPSNQTL KDYLSWRQAD CHINNLYNTV FWALIQQSGL TPVQAQGRLQ GTLAADKNEI LFSEFNINYN NELPMYRKGT VLIWQKVDEV MTKEIKLPTE MEGKKMAVTR TRTKPVPLHC DIIGDAFWKE HPEILDEDS

Clonality

Monoclonal

Isotype

IgG2a,k

Clone Number

4F11

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human THG1L.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

34,831 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (157158323..157166982). Location: 5q33.3

3D Structure

ModBase 3D Structure for Q9NWX6

Immunogen

Full length recombinant corresponding to aa1-270 from human THG1L (AAH01523) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-THG1L antibody

Antibodies; Abs to Enzymes; Transferase

Applications Tested/Suitable for anti-THG1L antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-THG1L antibody

Suitable for use in ELISA and Western Blot.

Other Notes

Small volumes of anti-THG1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for THG1L purchase

MBS642601 is an antibody targeting THG1L. Also known as THG1L (Probable tRNA(His) Guanylyltransferase, tRNA-histidine Guanylyltransferase, IHG-1, Interphase Cytoplasmic Foci Protein 45, ICF45). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for THG1L. It may not necessarily be applicable to this product.

NCBI GI #

33989068

NCBI GeneID

54974

NCBI Accession #

AAH01523.2

UniProt Primary Accession #

Q9NWX6

UniProt Secondary Accession #

Q53G12; Q7L5R3; Q9H0S2; D3DQJ5

UniProt Related Accession #

Q9NWX6

NCBI Official Full Name

THG1L protein

NCBI Official Synonym Full Names

tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)

NCBI Official Symbol

THG1L

NCBI Official Synonym Symbols

ICF45; IHG-1

UniProt Protein Name

Probable tRNA(His) guanylyltransferase

UniProt Synonym Protein Names

Interphase cytoplasmic foci protein 45; tRNA-histidine guanylyltransferase

UniProt Gene Name

THG1L

UniProt Synonym Gene Names

ICF45

UniProt Entry Name

THG1_HUMAN

NCBI Protein Information

probable tRNA(His) guanylyltransferase; probable tRNA(His) guanylyltransferase; induced by high glucose-1; interphase cytoplasmic foci protein 45

UniProt Comments for THG1L

ICF45: Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. Belongs to the tRNA(His) guanylyltransferase family.

Protein type: Transferase; Mitochondrial; EC 2.7.7.79; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: mitochondrion; cytoplasm

Molecular Function: identical protein binding; tRNA guanylyltransferase activity; GTP binding; magnesium ion binding; ATP binding; tRNA binding

Biological Process: tRNA processing; tRNA modification; protein homotetramerization

Research Articles on THG1L

1. IHG-1 increases mitochondrial biogenesis by promoting PGC-1alpha-dependent processes, potentially contributing to the pathogenesis of renal fibrosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.