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CA14 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642712

CA14 belongs a large family of zinc metalloenzymes that catalyze the versible hydration of carbon dioxide. The protein is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII;… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CA14, Polyclonal Antibody
Full Product Name
CA14, NT (CA14, Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV)
Product Gene Name
Product Synonym Names
Anti -CA14, NT (CA14, Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
37,668 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (150229554..150237478). Location: 1q21
OMIM
604832
3D Structure
ModBase 3D Structure for Q9ULX7
Immunogen
CA14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids from the N-terminal region of human CA14.

Product Categories/Family for anti-CA14 antibody

Applications Tested/Suitable for anti-CA14 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-CA14 antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-CA14 antibody

Western blot analysis of CA14 antibody (N-term) in A375 cell line lysates (35ug/lane). CA14 (arrow) was detected using the purified Pab.
Rabbit Anti-CA14 Polyclonal Antibody – Cat# MBS642712

Flow Cytometry (FC/FACS) of anti-CA14 antibody

Flow cytometric analysis of A375 cells using CA14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CA14 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS642712

Western Blot (WB) of anti-CA14 antibody

Western blot analysis of CA14 (arrow) using rabbit polyclonal CA14 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene.
Rabbit Anti-CA14 Polyclonal Antibody, Western Blot (WB) – Cat# MBS642712

Other Notes

Small volumes of anti-CA14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CA14 purchase

MBS642712 is an antibody targeting CA14. Also known as CA14, NT (CA14, Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CA14. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carbonic anhydrase 14
NCBI Official Synonym Full Names
carbonic anhydrase XIV
NCBI Official Symbol
CA14
NCBI Official Synonym Symbols
CAXiV
UniProt Protein Name
Carbonic anhydrase 14
UniProt Synonym Protein Names
Carbonate dehydratase XIV; Carbonic anhydrase XIV
Protein Family
UniProt Gene Name
CA14
UniProt Synonym Gene Names
CA-XIV
UniProt Entry Name
CAH14_HUMAN
NCBI Protein Information
carbonic anhydrase 14; CA-XIV; carbonic dehydratase; carbonate dehydratase XIV

NCBI Summary for CA14

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq, Jul 2008]
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UniProt Comments for CA14

CA14: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; EC 4.2.1.1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbonate dehydratase activity; metal ion binding

Biological Process: bicarbonate transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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