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OTX1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642737

This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
OTX1, Monoclonal Antibody
Full Product Name
OTX1 (Homeobox Protein OTX1, Orthodenticle Homolog 1, FLJ38361, MGC15736)
Product Synonym Names
Anti -OTX1 (Homeobox Protein OTX1, Orthodenticle Homolog 1, FLJ38361, MGC15736)
Immunogen Sequence
YGMNGLGLAG PAMDLLHPSV GYPATPRKQR RERTTFTRSQ LDVLEALFAK TRYPDIFMRE EVALKINLPE SRVQVWFKNR RAKCRQQQQS GSGTKSRPAK KKSSPVR
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1F2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human OTX1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa10-117 from human OTX1 (NP_055377) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-OTX1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-OTX1 antibody
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 10ug/ml

Western Blot (WB) of anti-OTX1 antibody

Western Blot detection against Immunogen (37.51kD).
Mouse Anti-OTX1 Monoclonal Antibody – Cat# MBS642737

Western Blot (WB) of anti-OTX1 antibody

Western Blot analysis of OTX1 expression in transfected 293T cell line by OTX1 monoclonal antibody.|Lane 1: OTX1 transfected lysate (37.3kD).|Lane 2: Non-transfected lysate.
Mouse Anti-OTX1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS642737

Immunofluorescence (IF) of anti-OTX1 antibody

Immunofluorescence of monoclonal antibody to OTX1 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-OTX1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS642737

Testing Data of anti-OTX1 antibody

Detection limit for recombinant GST tagged OTX1 is ~0.03ng/ml as a capture antibody.
Mouse Anti-OTX1 Monoclonal Antibody, Testing Data – Cat# MBS642737

Western Blot (WB) of anti-OTX1 antibody

Western blot analysis of OTX1 over-expressed 293 cell line, cotransfected with OTX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with OTX1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-OTX1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS642737

Other Notes

Small volumes of anti-OTX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OTX1 purchase

MBS642737 is an antibody targeting OTX1. Also known as OTX1 (Homeobox Protein OTX1, Orthodenticle Homolog 1, FLJ38361, MGC15736). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for OTX1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
Otx1
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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