Rat Anti-LAG3 Monoclonal Antibody – Cat# MBS642763

Detection of LAG-3 in mouse pplenocytes by Flow Cytometry. Mouse splenocytes were treated for 72h with 50ng/ml PMA and 500ng/ml Ca2+ Ionomycin then stained with MBS642763 or isotype control antibody, followed by Allophycocyanin- conjugated Rat IgG Secondary Antibody.

LAG3 Antibody – Rat Monoclonal

Name: LAG3 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS642763
Price: 1250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642763

LAG-3 is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496aa membrane protein with a 421aa extracellular region which contains four IgSF domains, a 21aa transmembrane region and a 54aa cytoplasmic region. LAG-3 shares More details

$1250 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

LAG3, Monoclonal Antibody

Full Product Name

LAG3 (Lymphocyte Activation Gene 3 Protein, LAG-3, Protein FDC, CD223, FDC)

Product Gene Name

anti-LAG3 antibody

Product Synonym Names

Anti -LAG3 (Lymphocyte Activation Gene 3 Protein, LAG-3, Protein FDC, CD223, FDC)

Clonality

Monoclonal

Isotype

IgG2B

Clone Number

12K124

Host

Rat

Species Reactivity

Mouse

Form/Format

Supplied as a lyophilized powder from PBS pH7.4, 5% trehalose. Reconstitute with 200ul sterile PBS

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Concentration

~0.5mg/ml after reconstitution (lot specific)

Specificity

Recognizes mouse LAG-3. Does not react with recombinant human LAG-3.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chromosome Location

Chromosome: 12; NC_000012.11 (6881670..6887621). Location: 12p13.32

OMIM

153337

3D Structure

ModBase 3D Structure for P18627

Immunogen

Recombinant preotein corresponding to Ser23-Leu442 from mouse LAG-3 expressed in NS0 cell line.

Accession No

Q61790

Product Categories/Family for anti-LAG3 antibody

Antibodies; Abs to Growth Factors; Cytokines

Applications Tested/Suitable for anti-LAG3 antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-LAG3 antibody

Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 2.5ug labels10e6 cells

Other Notes

Small volumes of anti-LAG3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LAG3 purchase

MBS642763 is an antibody targeting LAG3. Also known as LAG3 (Lymphocyte Activation Gene 3 Protein, LAG-3, Protein FDC, CD223, FDC). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for LAG3. It may not necessarily be applicable to this product.

NCBI GI #

167614500

NCBI GeneID

3902

NCBI Accession #

NP_002277.4

UniProt Primary Accession #

P18627

UniProt Secondary Accession #

A8K7T9

UniProt Related Accession #

P18627

GenBank Nucleotide #

NM_002286.5

NCBI Official Full Name

lymphocyte activation gene 3 protein

NCBI Official Synonym Full Names

lymphocyte-activation gene 3

NCBI Official Symbol

LAG3

NCBI Official Synonym Symbols

CD223

UniProt Protein Name

Lymphocyte activation gene 3 protein

UniProt Synonym Protein Names

Protein FDC

Protein Family

Lymphocyte activation gene 3 protein

UniProt Gene Name

LAG3

UniProt Synonym Gene Names

FDC; LAG-3

UniProt Entry Name

LAG3_HUMAN

NCBI Protein Information

lymphocyte activation gene 3 protein

NCBI Summary for LAG3

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

UniProt Comments for LAG3

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: transmembrane receptor activity; antigen binding; MHC class II protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation

Research Articles on LAG3

1. Expression of LAG-3 is coincident with the impaired effector function of HBV-specific CD8(+) T cell in HCC patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LAG3 antibody

Reference Product	PubMed Publications
CD4 antibody	>25 publications with LAG3 and CD4
FOXP3 antibody	>13 publications with LAG3 and FOXP3
CTLA4 antibody	>7 publications with LAG3 and CTLA4
PDCD1 antibody	>2 publications with LAG3 and PDCD1

Diseases associated with anti-LAG3 antibody

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>22 publications with LAG3 and Immune System Diseases
Neoplasms Antibodies	>9 publications with LAG3 and Neoplasms
Inflammation Antibodies	>8 publications with LAG3 and Inflammation
Disease Models, Animal Antibodies	>7 publications with LAG3 and Disease Models, Animal
Necrosis Antibodies	>3 publications with LAG3 and Necrosis
Immunologic Deficiency Syndromes Antibodies	>2 publications with LAG3 and Immunologic Deficiency Syndromes
Lymphoma Antibodies	>1 publications with LAG3 and Lymphoma
Breast Neoplasms Antibodies	>1 publications with LAG3 and Breast Neoplasms
Coronary Disease Antibodies	>1 publications with LAG3 and Coronary Disease
Lung Neoplasms Antibodies	>1 publications with LAG3 and Lung Neoplasms

Organs/Tissues associated with anti-LAG3 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>36 publications with LAG3 and Blood
Spleen Antibodies	>6 publications with LAG3 and Spleen
Intestine Antibodies	>2 publications with LAG3 and Intestine
Uterus Antibodies	>1 publications with LAG3 and Uterus
Brain Antibodies	>1 publications with LAG3 and Brain