• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

RHOJ Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642767

ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RHOJ, Polyclonal Antibody
Full Product Name
RHOJ, ID (RHOJ, ARHJ, RASL7B, RHOI, TCL, Rho-related GTP-binding protein RhoJ, Ras-like protein family member 7B, Tc10-like GTP-binding protein)
Product Gene Name
anti-RHOJ antibody
Product Synonym Names
Anti -RHOJ, ID (RHOJ, ARHJ, RASL7B, RHOI, TCL, Rho-related GTP-binding protein RhoJ, Ras-like protein family member 7B, Tc10-like GTP-binding protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
23,821 Da
Chromosome Location
Chromosome: 14; NC_000014.8 (63671080..63760230). Location: 14q23.2
OMIM
607653
3D Structure
ModBase 3D Structure for Q9H4E5
Immunogen
RHOJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the Central region of human RHOJ.

Product Categories/Family for anti-RHOJ antibody

Antibodies; Abs to Binding Proteins
Applications Tested/Suitable for anti-RHOJ antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-RHOJ antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Immunohistochemistry (IHC) of anti-RHOJ antibody

RHOJ Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RHOJ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-RHOJ Polyclonal Antibody – Cat# MBS642767

Western Blot (WB) of anti-RHOJ antibody

RHOJ Antibody (Center) western blot analysis in HepG2,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RHOJ antibody detected the RHOJ protein (arrow).
Rabbit Anti-RHOJ Polyclonal Antibody, Western Blot (WB) – Cat# MBS642767

Other Notes

Small volumes of anti-RHOJ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RHOJ purchase

MBS642767 is an antibody targeting RHOJ. Also known as RHOJ, ID (RHOJ, ARHJ, RASL7B, RHOI, TCL, Rho-related GTP-binding protein RhoJ, Ras-like protein family member 7B, Tc10-like GTP-binding protein). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RHOJ. It may not necessarily be applicable to this product.

NCBI GI #
16903164
NCBI GeneID
57381
NCBI Accession #
NP_065714.1
UniProt Primary Accession #
Q9H4E5
UniProt Secondary Accession #
Q96KC1
UniProt Related Accession #
Q9H4E5
GenBank Nucleotide #
NM_020663.4
NCBI Official Full Name
rho-related GTP-binding protein RhoJ
NCBI Official Synonym Full Names
ras homolog family member J
NCBI Official Symbol
RHOJ
NCBI Official Synonym Symbols
TCL; ARHJ; TC10B; RASL7B
UniProt Protein Name
Rho-related GTP-binding protein RhoJ
UniProt Synonym Protein Names
Ras-like protein family member 7B; Tc10-like GTP-binding protein
Protein Family
Rho-related GTP-binding protein
UniProt Gene Name
RHOJ
UniProt Synonym Gene Names
ARHJ; RASL7B; RHOI; TCL
UniProt Entry Name
RHOJ_HUMAN
NCBI Protein Information
rho-related GTP-binding protein RhoJ; TC10-like Rho GTPase; RAS-like, family 7, member B; tc10-like GTP-binding protein; ras homolog gene family, member J; ras-like protein family member 7B

NCBI Summary for RHOJ

This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011]
Show more

UniProt Comments for RHOJ

RHOJ: GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology. Belongs to the small GTPase superfamily. Rho family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: plasma membrane; cytosol

Molecular Function: GTPase activity; GTP binding

Biological Process: regulation of cell shape; regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; actin cytoskeleton organization and biogenesis; Rho protein signal transduction
Show more

Research Articles on RHOJ

1. These observations identify RHOJ as a melanoma linchpin determinant that regulates both actin cytoskeletal dynamics and chemoresistance by activating PAK1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RHOJ antibody

 Reference Product  PubMed Publications
 CDC42 antibody  >10 publications with RHOJ and CDC42
 PAK1 antibody  >3 publications with RHOJ and PAK1

Pathways associated with anti-RHOJ antibody

 Products by Pathway  Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Rho GTPase Cycle Pathway antibodies  Rho GTPase Cycle Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By Rho GTPases Pathway antibodies  Signaling By Rho GTPases Pathway Diagram

Diseases associated with anti-RHOJ antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5 publications with RHOJ and Neoplasms
 Carcinoma Antibodies  >2 publications with RHOJ and Carcinoma
 Skin Neoplasms Antibodies  >1 publications with RHOJ and Skin Neoplasms
 Disease Models, Animal Antibodies  >1 publications with RHOJ and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >1 publications with RHOJ and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >1 publications with RHOJ and Neoplasms, Experimental
 Inflammation Antibodies  >1 publications with RHOJ and Inflammation
 Necrosis Antibodies  >1 publications with RHOJ and Necrosis
 Cardiomyopathies Antibodies  >1 publications with RHOJ and Cardiomyopathies

Organs/Tissues associated with anti-RHOJ antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >7 publications with RHOJ and Vascular
 Heart Antibodies  >6 publications with RHOJ and Heart
 Blood Antibodies  >4 publications with RHOJ and Blood
 Embryonic Tissue Antibodies  >2 publications with RHOJ and Embryonic Tissue
 Eye Antibodies  >2 publications with RHOJ and Eye
 Lung Antibodies  >2 publications with RHOJ and Lung
 Brain Antibodies  >2 publications with RHOJ and Brain
 Stomach Antibodies  >1 publications with RHOJ and Stomach
 Skin Antibodies  >1 publications with RHOJ and Skin
 Uterus Antibodies  >1 publications with RHOJ and Uterus
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000