• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TFAM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS642889

This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TFAM, Polyclonal Antibody
Full Product Name
TFAM, CT (TFAM, TCF6, TCF6L2, Transcription factor A, mitochondrial, Mitochondrial transcription factor 1, Transcription factor 6, Transcription factor 6-like 2)
Product Gene Name
anti-TFAM antibody
Product Synonym Names
Anti -TFAM, CT (TFAM, TCF6, TCF6L2, Transcription factor A, mitochondrial, Mitochondrial transcription factor 1, Transcription factor 6, Transcription factor 6-like 2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
29,097 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (60144903..60158990). Location: 10q21
OMIM
600438
3D Structure
ModBase 3D Structure for Q00059
Immunogen
TFAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-254 amino acids from the C-terminal region of human TFAM.

Product Categories/Family for anti-TFAM antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-TFAM antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-TFAM antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50

Immunohistochemistry (IHC) of anti-TFAM antibody

TFAM antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TFAM antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-TFAM Polyclonal Antibody – Cat# MBS642889

Flow Cytometry (FC/FACS) of anti-TFAM antibody

TFAM Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-TFAM Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS642889

Immunofluorescence (IF) of anti-TFAM antibody

Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody (C-term). NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TFAM immunoreactivity is localized to mitochondrion significantly.
Rabbit Anti-TFAM Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS642889

Western Blot (WB) of anti-TFAM antibody

TFAM Antibody (C-term) western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the TFAM antibody detected the TFAM protein (arrow).
Rabbit Anti-TFAM Polyclonal Antibody, Western Blot (WB) – Cat# MBS642889

Other Notes

Small volumes of anti-TFAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFAM purchase

MBS642889 is an antibody targeting TFAM. Also known as TFAM, CT (TFAM, TCF6, TCF6L2, Transcription factor A, mitochondrial, Mitochondrial transcription factor 1, Transcription factor 6, Transcription factor 6-like 2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TFAM. It may not necessarily be applicable to this product.

NCBI GI #
399498566
NCBI GeneID
7019
NCBI Accession #
NP_001257711.1
UniProt Primary Accession #
Q00059
UniProt Secondary Accession #
Q5U0C6; A8MRB2; A9QXC6; B5BU05
UniProt Related Accession #
Q00059
GenBank Nucleotide #
NM_001270782.1
NCBI Official Full Name
transcription factor A, mitochondrial isoform 2
NCBI Official Synonym Full Names
transcription factor A, mitochondrial
NCBI Official Symbol
TFAM
NCBI Official Synonym Symbols
TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
UniProt Protein Name
Transcription factor A, mitochondrial
UniProt Synonym Protein Names
Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2
Protein Family
Transcription factor
UniProt Gene Name
TFAM
UniProt Synonym Gene Names
TCF6; TCF6L2; mtTFA; MtTF1; TCF-6
UniProt Entry Name
TFAM_HUMAN
NCBI Protein Information
transcription factor A, mitochondrial; transcription factor 6; transcription factor 6-like 1; transcription factor 6-like 3; mitochondrial transcription factor 1; mitochondrial transcription factor A; transcription factor 6-like 2 (mitochondrial transcription factor)

NCBI Summary for TFAM

This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Show more

UniProt Comments for TFAM

TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.

Protein type: Transcription regulation; DNA-binding

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: mitochondrion; mitochondrial matrix; nucleus; cytosol

Molecular Function: protein binding; heat shock protein binding; chromatin binding; transcription factor activity; DNA bending activity

Biological Process: chromatin remodeling; mitochondrial respiratory chain complex assembly; mitochondrion organization and biogenesis; regulation of transcription from RNA polymerase I promoter; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; gene expression; positive regulation of transcription from RNA polymerase II promoter; DNA-dependent DNA replication; transcription initiation from mitochondrial promoter; transcription from mitochondrial promoter
Show more

Research Articles on TFAM

1. In cells with normal mitochondrial DNA levels, phosphorylated TFAM is degraded by Lon.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TFAM antibody

 Reference Product  PubMed Publications
 NRF1 antibody  >80 publications with TFAM and NRF1
 TFB2M antibody  >33 publications with TFAM and TFB2M
 TFB1M antibody  >23 publications with TFAM and TFB1M
 POLRMT antibody  >14 publications with TFAM and POLRMT
 GABPA antibody  >10 publications with TFAM and GABPA
 TXN2 antibody  >1 publications with TFAM and TXN2

Pathways associated with anti-TFAM antibody

 Products by Pathway  Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Mitochondrial Gene Expression Pathway antibodies  Mitochondrial Gene Expression Pathway Diagram
 Mitochondrial Transcription Initiation Pathway antibodies  Mitochondrial Transcription Initiation Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 Transcription From Mitochondrial Promoters Pathway antibodies  Transcription From Mitochondrial Promoters Pathway Diagram

Diseases associated with anti-TFAM antibody

 Disease Name  Pubmed Publications
 Mitochondrial Diseases Antibodies  >79 publications with TFAM and Mitochondrial Diseases
 Nervous System Diseases Antibodies  >64 publications with TFAM and Nervous System Diseases
 Disease Models, Animal Antibodies  >44 publications with TFAM and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >41 publications with TFAM and Cardiovascular Diseases
 Insulin Resistance Antibodies  >37 publications with TFAM and Insulin Resistance
 Heart Diseases Antibodies  >31 publications with TFAM and Heart Diseases
 Inflammation Antibodies  >25 publications with TFAM and Inflammation
 Necrosis Antibodies  >23 publications with TFAM and Necrosis
 Hyperinsulinism Antibodies  >19 publications with TFAM and Hyperinsulinism
 Liver Diseases Antibodies  >15 publications with TFAM and Liver Diseases

Organs/Tissues associated with anti-TFAM antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >171 publications with TFAM and Muscle
 Heart Antibodies  >79 publications with TFAM and Heart
 Liver Antibodies  >65 publications with TFAM and Liver
 Blood Antibodies  >59 publications with TFAM and Blood
 Brain Antibodies  >56 publications with TFAM and Brain
 Connective Tissue Antibodies  >24 publications with TFAM and Connective Tissue
 Vascular Antibodies  >15 publications with TFAM and Vascular
 Thyroid Antibodies  >15 publications with TFAM and Thyroid
 Lung Antibodies  >14 publications with TFAM and Lung
 Kidney Antibodies  >13 publications with TFAM and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000