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BHLHE22 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643072

BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence… More details

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Product Overview

Online Datasheet in PDF
Product Name
BHLHB5 (BHLHE22), Polyclonal Antibody
Full Product Name
BHLHB5, ID (BHLHE22, BHLHB5, TNRC20, Class E basic helix-loop-helix protein 22, Class B basic helix-loop-helix protein 5, Trinucleotide repeat-containing gene 20 protein)
Product Gene Name
anti-BHLHE22 antibody
Product Synonym Names
Anti -BHLHB5, ID (BHLHE22, BHLHB5, TNRC20, Class E basic helix-loop-helix protein 22, Class B basic helix-loop-helix protein 5, Trinucleotide repeat-containing gene 20 protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
36,997 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (65492795..65496191). Location: 8q13
OMIM
613483
3D Structure
ModBase 3D Structure for Q8NFJ8
Immunogen
BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243~272 amino acids from the Central region of human BHLHB5.

Product Categories/Family for anti-BHLHE22 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-BHLHE22 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-BHLHE22 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-BHLHE22 antibody

Western blot analysis of BHLHB5 Antibody (Center) in HL-60 cell line lysates (35ug/lane). BHLHB5 (arrow) was detected using the purified Pab.
Rabbit Anti-BHLHE22 Polyclonal Antibody – Cat# MBS643072

Immunohistochemistry (IHC) of anti-BHLHE22 antibody

BHLHB5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BHLHB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-BHLHE22 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS643072

Flow Cytometry (FC/FACS) of anti-BHLHE22 antibody

BHLHB5 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-BHLHE22 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS643072

Other Notes

Small volumes of anti-BHLHE22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BHLHE22 purchase

MBS643072 is an antibody targeting BHLHE22. Also known as BHLHB5, ID (BHLHE22, BHLHB5, TNRC20, Class E basic helix-loop-helix protein 22, Class B basic helix-loop-helix protein 5, Trinucleotide repeat-containing gene 20 protein). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BHLHE22. It may not necessarily be applicable to this product.

NCBI GI #
119607282
NCBI GeneID
27319
NCBI Accession #
EAW86876.1
UniProt Primary Accession #
Q8NFJ8
UniProt Related Accession #
Q8NFJ8
NCBI Official Full Name
basic helix-loop-helix domain containing, class B, 5
NCBI Official Synonym Full Names
basic helix-loop-helix family, member e22
NCBI Official Symbol
BHLHE22
NCBI Official Synonym Symbols
Beta3; BHLHB5; CAGL85; TNRC20
UniProt Protein Name
Class E basic helix-loop-helix protein 22
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 5; bHLHb5; Trinucleotide repeat-containing gene 20 protein
Protein Family
Class E basic helix-loop-helix protein
UniProt Gene Name
BHLHE22
UniProt Synonym Gene Names
BHLHB5; TNRC20; bHLHe22; bHLHb5
UniProt Entry Name
BHE22_HUMAN
NCBI Protein Information
class E basic helix-loop-helix protein 22; trinucleotide repeat containing 20; class B basic helix-loop-helix protein 5; trinucleotide repeat-containing gene 20 protein; basic helix-loop-helix domain containing, class B, 5

NCBI Summary for BHLHE22

BHLHE22 belongs to the basic helix-loop-helix (bHLH) family of transcription factors that regulate cell fate determination, proliferation, and differentiation. These proteins function as dimers and bind to an E-box DNA sequence (CANNTG). BHLHE22 is expressed exclusively in the central nervous system and retina (Xu et al., 2002 [PubMed 12213201]).[supplied by OMIM, Jul 2010]
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UniProt Comments for BHLHE22

BHLHB5: Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; anterior commissure morphogenesis; corpus callosum morphogenesis; cerebral cortex regionalization; corticospinal tract morphogenesis; negative regulation of transcription, DNA-dependent; retinal bipolar neuron differentiation
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Research Articles on BHLHE22

1. Segregates with Duane syndrome. Brain-specific expression with the highest abundance in the cerebellum.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-BHLHE22 antibody

 Disease Name  Pubmed Publications
 Nervous System Malformations Antibodies  >1 publications with BHLHE22 and Nervous System Malformations
 Eye Abnormalities Antibodies  >1 publications with BHLHE22 and Eye Abnormalities
 Inflammation Antibodies  >1 publications with BHLHE22 and Inflammation
 Nervous System Diseases Antibodies  >1 publications with BHLHE22 and Nervous System Diseases

Organs/Tissues associated with anti-BHLHE22 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with BHLHE22 and Brain
 Eye Antibodies  >4 publications with BHLHE22 and Eye
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