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ALDH1A1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643330

ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ALDH1A1, Polyclonal Antibody
Full Product Name
ALDH1A1, ID (ALDH1A1, ALDC, ALDH1, PUMB1, Retinal dehydrogenase 1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic)
Product Synonym Names
Anti -ALDH1A1, ID (ALDH1A1, ALDC, ALDH1, PUMB1, Retinal dehydrogenase 1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ALDH1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309~339 amino acids from the center region of human ALDH1A1.
Applications Tested/Suitable for anti-ALDH1A1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-ALDH1A1 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunofluorescence: 1:10-50
Immunohistochemistry: 1:50
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-ALDH1A1 antibody

Western Blot analysis of 293 cell lysates (2ug/lane) using MBS643330 in either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).
Rabbit Anti-ALDH1A1 Polyclonal Antibody – Cat# MBS643330

Western Blot (WB) of anti-ALDH1A1 antibody

Western Blot analysis in human liver lysate (20ug) using MBS643330 (1:1000). Secondary IgG (HRP) Goat Anti-Rabbit. Predicted band size: 55kD.
Rabbit Anti-ALDH1A1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS643330

Immunohistochemistry (IHC) of anti-ALDH1A1 antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue using MBS643330, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Rabbit Anti-ALDH1A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS643330

Immunofluorescence (IF) of anti-ALDH1A1 antibody

Immunofluorescence analysis of HeLa cells using MBS643330 (0.025mg/ml) followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Rabbit Anti-ALDH1A1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS643330

Other Notes

Small volumes of anti-ALDH1A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALDH1A1 purchase

MBS643330 is an antibody targeting ALDH1A1. Also known as ALDH1A1, ID (ALDH1A1, ALDC, ALDH1, PUMB1, Retinal dehydrogenase 1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ALDH1A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
Aldh1a1
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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