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PRAMEF20 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643347

Melanoma antigen preferentially expressed in tumors is a protein that in humans is encoded by the PRAME gene.1,2,3 which encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRAMEF20, Polyclonal Antibody
Full Product Name
PRAMEF20, CT (PRAMEF20, PRAME family member 20/21)
Product Gene Name
Product Synonym Names
Anti -PRAMEF20, CT (PRAMEF20, PRAME family member 20/21)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
54,770 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (13736907..13747803). Location: 1p36.21
3D Structure
ModBase 3D Structure for Q5VT98
Immunogen
PRAMEF20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-431 amino acids from the C-terminal region of human PRAMEF20.
Applications Tested/Suitable for anti-PRAMEF20 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-PRAMEF20 antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-PRAMEF20 antibody

PRAMEF20 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PRAMEF20 antibody detected the PRAMEF20 protein (arrow).
Rabbit Anti-PRAMEF20 Polyclonal Antibody – Cat# MBS643347

Other Notes

Small volumes of anti-PRAMEF20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRAMEF20 purchase

MBS643347 is an antibody targeting PRAMEF20. Also known as PRAMEF20, CT (PRAMEF20, PRAME family member 20/21). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PRAMEF20. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
PRAME family member 20/21
NCBI Official Synonym Full Names
PRAME family member 20
NCBI Official Symbol
PRAMEF20
UniProt Protein Name
PRAME family member 20/21
Protein Family
UniProt Gene Name
PRAMEF20
UniProt Entry Name
PRA20_HUMAN
NCBI Protein Information
PRAME family member 20/21

UniProt Comments for PRAMEF20

PRAMEF20: Belongs to the PRAME family.

Chromosomal Location of Human Ortholog: 1p36.21

Molecular Function: retinoic acid receptor binding

Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell differentiation; positive regulation of cell proliferation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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