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WAPAL Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643436

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
WAPAL, Monoclonal Antibody
Full Product Name
WAPAL (Wings Apart-like Protein Homolog, WAPL, Friend of EBNA2 Protein, FOE, KIAA0261)
Product Gene Name
Product Synonym Names
Anti -WAPAL (Wings Apart-like Protein Homolog, WAPL, Friend of EBNA2 Protein, FOE, KIAA0261)
Immunogen Sequence
MDLDRASLDL MIRLLELEQD ASSAKLLNEK DMNKIKEKIR RLCETVHNKH LDLENITTGH LAMETLLSLT SKRAGDWFKE ELRLLGGLDH IVDKVKECVD HLSRDEDEEK LVASLWGAER CLRVLESVTV HNPENQSYLI AYKDSQLIVS SAKALQHCEE LIQQYNRAED SICLADSKPL PHQNVTNHVG KAVEDCMRAI IGVLLNLTND NEWGSTKTGE QDGLIGTALN CVLQVPKYLP QEQRFDIRVL LFLER
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2B3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human KIAA0261.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
132,946 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (88195013..88281541, complement). Location: 10q23.2
OMIM
610754
3D Structure
ModBase 3D Structure for Q7Z5K2
Immunogen
Full length recombinant corresponding to aa1-403 from KIAA0261 (AAH17393) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-WAPAL antibody

Applications Tested/Suitable for anti-WAPAL antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-WAPAL antibody
Suitable for use in ELISA, Immunoprecipitation and Western Blot.

Western Blot (WB) of anti-WAPAL antibody

Western Blot detection against Immunogen (70.33kD).
Mouse Anti-WAPAL Monoclonal Antibody – Cat# MBS643436

Western Blot (WB) of anti-WAPAL antibody

Western Blot analysis of KIAA0261 expression in transfected 293T cell line by KIAA0261 monoclonal antibody |Lane 1: KIAA0261 transfected lysate (45.6kD).|Lane 2: Non-transfected lysate.
Mouse Anti-WAPAL Monoclonal Antibody, Western Blot (WB) – Cat# MBS643436

Immunoprecipitation (IP) of anti-WAPAL antibody

Immunoprecipitation of WAPAL transfected lysate using WAPAL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WAPAL monoclonal antibody.
Mouse Anti-WAPAL Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS643436

Testing Data of anti-WAPAL antibody

Detection limit for recombinant GST tagged KIAA0261 is ~0.3ng/ml as a capture antibody.
Mouse Anti-WAPAL Monoclonal Antibody, Testing Data – Cat# MBS643436

Other Notes

Small volumes of anti-WAPAL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WAPAL purchase

MBS643436 is an antibody targeting WAPAL. Also known as WAPAL (Wings Apart-like Protein Homolog, WAPL, Friend of EBNA2 Protein, FOE, KIAA0261). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for WAPAL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
WAPAL protein
NCBI Official Synonym Full Names
wings apart-like homolog (Drosophila)
NCBI Official Symbol
WAPAL
NCBI Official Synonym Symbols
FOE; WAPL; KIAA0261
UniProt Protein Name
Wings apart-like protein homolog
UniProt Synonym Protein Names
Friend of EBNA2 protein
UniProt Gene Name
WAPAL
UniProt Synonym Gene Names
FOE; KIAA0261; WAPL
UniProt Entry Name
WAPL_HUMAN
NCBI Protein Information
wings apart-like protein homolog; friend of EBNA2 protein; friend of EBNA2 (Epstein-Barr virus nuclear protein 2)

UniProt Comments for WAPAL

WAPL: a chromatin-associated protein partner of Epstein-Barr nuclear protein 2. Associated with cervical carcinogenesis and tumor progression.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 10q23.2

Cellular Component: cohesin complex; nucleoplasm; microtubule cytoskeleton; intracellular membrane-bound organelle; cytoplasm; synaptonemal complex; chromosome; chromatin; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein binding

Biological Process: mitosis; positive regulation of fibroblast proliferation; viral reproduction; negative regulation of DNA replication; cell division; response to toxin; negative regulation of sister chromatid cohesion; mitotic cell cycle
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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