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PCAT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643535

PCAT1 acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to hosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PCAT1, Polyclonal Antibody
Full Product Name
PCAT1, CT (LPCAT1, AYTL2, PFAAP3, Lysophosphatidylcholine acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-lik
Product Synonym Names
Anti -PCAT1, CT (LPCAT1, AYTL2, PFAAP3, Lysophosphatidylcholine acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-lik
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PCAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 505~534 amino acids from the C-terminal region of human PCAT1.
Applications Tested/Suitable for anti-PCAT1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-PCAT1 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-PCAT1 antibody

Western blot analysis of PCAT1 Antibody (C-term) in Hela cell line lysates (35ug/lane). PCAT1 (arrow) was detected using the purified Pab.
Rabbit Anti-PCAT1 Polyclonal Antibody – Cat# MBS643535

Immunohistochemistry (IHC) of anti-PCAT1 antibody

Formalin-fixed and paraffin-embedded human spleen tissue reacted with PCAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PCAT1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS643535

Flow Cytometry (FC/FACS) of anti-PCAT1 antibody

PCAT1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PCAT1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS643535

Other Notes

Small volumes of anti-PCAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCAT1 purchase

MBS643535 is an antibody targeting PCAT1. Also known as PCAT1, CT (LPCAT1, AYTL2, PFAAP3, Lysophosphatidylcholine acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-lik. Reactive in Human. Host: Rabbit.
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