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PRDM2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643679

This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene.… More details

$865 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRDM2, Monoclonal Antibody
Full Product Name
PRDM2 (RIZ1) (PRDM2, KMT8, RIZ, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger pro
Product Gene Name
Product Synonym Names
Anti -PRDM2 (RIZ1) (PRDM2, KMT8, RIZ, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger pro
Clonality
Monoclonal
Isotype
IgG1
Clone Number
33AT1045
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromosome Location
Chromosome: 4; NC_000070.6 (143107391..143212709, complement). Location: 4 E1; 4 76.84 cM
Immunogen
Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. antibody is specific for RIZ1, it does not recognize the isoform beta (RIZ2).
Applications Tested/Suitable for anti-Prdm2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-Prdm2 antibody
Suitable for use in Western Blot, Immunoprecipitation, ELISA.
Dilution: ELISA: 1:1,000
Western Blot: 1:100~500
Immuno precipitation

Western Blot (WB) of anti-Prdm2 antibody

Western blot analysis of PRDM2 in PRDM2-transgenic lysate. HRP-conjugated anti-mouse secondary antibody/chemiluminescence detection were used for signal visualization.
Mouse Anti-PRDM2 Monoclonal Antibody – Cat# MBS643679

Immunoprecipitation (IP) of anti-Prdm2 antibody

ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed using antibody MBS643679. DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.
Mouse Anti-PRDM2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS643679

Immunoprecipitation (IP) of anti-Prdm2 antibody

ChIP analysis of estrogen target genes. (B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed using antibody MBS643679 and control immunoglobulin G as indicated. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.
Mouse Anti-PRDM2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS643679

Immunoprecipitation (IP) of anti-Prdm2 antibody

ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts using antibody MBS643679. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in: Carling et al., 2004. Mol Cell Bio. 24 (16): 7032-7042.
Mouse Anti-PRDM2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS643679

Other Notes

Small volumes of anti-Prdm2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Prdm2 purchase

MBS643679 is an antibody targeting Prdm2. Also known as PRDM2 (RIZ1) (PRDM2, KMT8, RIZ, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger pro. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Prdm2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Official Full Name
Prdm2 protein
NCBI Official Synonym Full Names
PR domain containing 2, with ZNF domain
NCBI Official Symbol
Prdm2
NCBI Official Synonym Symbols
Riz; KMT8; Riz1; Gm1033; Gm19732; Znfpr1c1; 4833427P12Rik; E330024L24Rik
NCBI Protein Information
retinoblastoma protein-binding zinc finger protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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